It is an object of the present invention to provide a microscope that is capable of precisely controlling the amount of an immersion medium held in a gap between an objective lens and a mounting part, and that is capable of observing a specimen without varying the mounting part. The microscope includes a supply port from which an immersion medium is supplied to a gap between a film-like mounting part on which a specimen is loaded and an immersion objective lens, and a suction port that is arranged so as to be able to be brought into contact with an immersion medium supplied from the supply port, and from which the immersion medium can be sucked.
1. A method of using a microscope for observing a specimen loaded on a film-like mounting part mountable on a stage, the microscope comprising: a supply port which is adapted to be positioned to supply an immersion medium to a gap between the film-like mounting part mounted on the stage and an immersion objective lens which is arranged facing upward at a lower side of the stage; a suction port which is adapted to be positioned to contact the immersion medium suppliable from the supply port and to suck the immersion medium; a supply unit which is connected to the supply port and which is adapted to supply the immersion medium to the gap between the film-like mounting part and the immersion objective lens through the supply port; and a suction unit which is connected to the suction port and which is adapted to suck the immersion medium through the suction port, the method comprising:
operating the suction unit to suck a whole amount of the immersion medium provided in the gap between the film-like mounting part and the immersion objective lens; and
operating the supply unit to supply a predetermined amount of the immersion medium to the gap between the film-like mounting part and the immersion objective lens.
2. A microscope for observing a specimen loaded on a film-like mounting part mountable on a stage, the microscope comprising:
a supply port which is adapted to be positioned to supply an immersion medium to a gap between the film-like mounting part mounted on the stage and an immersion objective lens which is arranged facing upward at a lower side of the stage;
a suction port which is adapted to be positioned to contact the immersion medium suppliable from the supply port and to suck the immersion medium;
a supply unit which is connected to the supply port and which is adapted to supply the immersion medium to the gap between the film-like mounting part and the immersion objective lens through the supply port;
a suction unit which is connected to the suction port and which is adapted to suck the immersion medium through the suction port; and
a control unit which is adapted to drive the supply unit and the suction unit,
wherein the control unit is adapted to operate the suction unit to suck a whole amount of the immersion medium provided in the gap between the film-like mounting part and the immersion objective lens, and thereafter, operate the supply unit to supply a predetermined amount of the immersion medium to the gap between the film-like mounting part and the immersion objective lens.
3. A non-transitory computer-readable recording medium storing a program of a microscope for observing a specimen loaded on a film-like mounting part mountable on a stage, wherein the microscope comprises: a supply port which is adapted to be positioned to supply an immersion medium to a gap between the film-like mounting part mounted on the stage and an immersion objective lens which is arranged facing upward at a lower side of the stage; a suction port which is adapted to be positioned to contact the immersion medium suppliable from the supply port and to suck the immersion medium; a supply unit which is connected to the supply port and which is adapted to supply the immersion medium to the gap between the film-like mounting part and the immersion objective lens through the supply port; and a suction unit which is connected to the suction port and which is adapted to suck the immersion medium through the suction port, the program causing a computer of the microscope to perform functions comprising:
operating the suction unit to suck a whole amount of the immersion medium provided in the gap between the film-like mounting part and the immersion objective lens; and
operating the supply unit to supply a predetermined amount of the immersion medium to the gap between the film-like mounting part and the immersion objective lens.