Purple transgenic fluorescent ornamental fish
View Patent ↗The present invention relates to transgenic purple ornamental fish, as well as methods of making such fish by in vitro fertilization techniques. Also disclosed are methods of establishing a population of such transgenic fish and methods of providing them to the ornamental fish industry for the purpose of marketing.
1. A transgenic tetra comprising a chromosomally integrated expression cassette encoding a fluorescent protein, wherein the tetra comprises the “Purple tetra 1 transformation event,” sperm comprising the Purple tetra 1 transformation event having been deposited as ECACC accession no. 14011702.
2. The transgenic tetra of claim 1 , further defined as a fertile, transgenic tetra.
3. The transgenic tetra of claim 1 , further defined as a transgenic White Tetra.
4. The transgenic tetra of claim 1 , wherein the fish is homozygous for the integrated expression cassette.
5. The transgenic tetra of claim 1 , wherein the fish is heterozygous for the integrated expression cassette.
6. A method of providing a transgenic tetra to the ornamental fish market, comprising obtaining a transgenic tetra in accordance with claim 1 , and distributing the fish to the ornamental fish market.
7. The method of claim 6 , wherein the fish are distributed by a grower to a commercial distributor.
8. The method of claim 6 , wherein the fish are distributed by a grower or a commercial distributor to a retailer.
9. The method of claim 8 , wherein the retailer is a multi-product retailer having an ornamental fish department.
10. A method of producing a transgenic tetra comprising:
(a) obtaining a tetra that comprises a chromosomally integrated expression cassette encoding a fluorescent protein, wherein the tetra comprises the “Purple tetra 1 transformation event,” sperm comprising the Purple tetra 1 transformation event having been deposited as ECACC accession no. 14011702; and
(b) breeding the obtained tetra with a second tetra to provide a transgenic tetra comprising the Purple tetra 1 transformation event.
11. The method of claim 10 , wherein the second tetra is a non-transgenic tetra.
12. A progeny of a transgenic tetra of claim 1 that comprises a chromosomally integrated expression cassette encoding a fluorescent protein, wherein the tetra and progeny exhibit fluorescence and comprise the “Purple tetra 1 transformation event,” sperm comprising the Purple tetra 1 transformation event having been deposited as ECACC accession no. 14011702.
13. The progeny fish of claim 12 , further defined as a fertile, transgenic tetra.
14. The progeny fish of claim 12 , further defined as a transgenic White Tetra.
15. The progeny fish of claim 12 , wherein the fish is homozygous for the integrated expression cassette.
16. The progeny fish of claim 12 , wherein the fish is heterozygous for the integrated expression cassette.
17. A method of providing a transgenic fish to the ornamental fish market, comprising obtaining a progeny fish in accordance with claim 12 , and distributing the fish to the ornamental fish market.
18. The method of claim 17 , wherein the fish are distributed by a grower to a commercial distributor.
19. The method of claim 17 , wherein the fish are distributed by a grower or a commercial distributor to a retailer.
20. The method of claim 19 , wherein the retailer is a multi-product retailer having an ornamental fish department.
21. A method of producing a transgenic fish comprising:
(a) obtaining a transgenic fish in accordance with claim 12 ; and
(b) breeding the obtained fish with a second fish to provide a transgenic fish comprising the Purple tetra 1 transformation event.
22. The method of claim 21 , wherein the second fish is a non-transgenic fish.