Hollow fiber module having TFC-aquaporin modified membranes
The present invention relates to a hollow fiber (HF) module having fibers modified with a thin film composite (TFC) layer comprising aquaporin water channels.
1. A hollow porous fiber (HF) module having fibers modified with a thin film composite (TFC) layer comprising aquaporin water channels, wherein the TFC layer is a polyamide layer provided on the inside of the fibers.
2. The HF module according to claim 1 , wherein said aquaporin water channels are selected from the group consisting of AqpZ channels and SoPIP2;1 water channels.
3. The HF module according to claim 1 , wherein the fiber area is from about 0.1 cm 2 to about 0.5 m 2 .
4. The HF module of claim 1 , wherein said aquaporin water channels are incorporated in vesicles before incorporation into the TFC layer.
5. The HF module of claim 4 , wherein said vesicles into which the aquaporin water channels are incorporated are liposomes or polymersomes.
6. The HF module of claim 5 , wherein said liposomes are prepared from lipids selected from the group consisting of DPhPC, DOPC, mixed soy bean lipids, asolectin and E. coli mixed lipids.
7. The HF module of claim 6 , wherein said polymersomes comprise triblock copolymers of the hydrophile-hydrophobe-hydrophile (A-B-A or A-B-C) type, diblock copolymers of the hydrophile-hydrophobe type (A-B), or a combination thereof.
8. The HF module according to claim 4 , wherein said TFC layer is formed through interfacial polymerization of an aqueous solution of a di- or triamine with a solution of di- or triacyl halide in an organic solvent, and wherein the aquaporin water channel vesicles are incorporated in said aqueous solution.
9. The HF module of claim 1 , wherein the fibers are polyethersulfone fibers.
10. A method of preparing a hollow fiber module comprising porous HF membranes modified on the inside of the fibers with a thin film composite (TFC) layer comprising aquaporin water channels, said method comprising the steps of:
a) obtaining an aquaporin vesicles suspension, wherein said vesicles are proteoliposomes or proteopolymersomes having from about 25 to about 500 LPR/POPR of protein,
b) preparing an aqueous solution of a di- or triamine,
c) dissolving a di- or triacyl halide in an apolar organic solvent,
d) preparing a mixture of amine and aquaporin vesicle by dissolving/mixing the vesicles preparation from step a) with the solution from step b),
e) pumping the mixture from step d) through the lumen of the hollow fibers in a hollow fiber module using its end inlet,
f) removing excess aqueous solution by a gas purging of the lumen side of the fibers using a module inlet,
g) injecting the acyl halide solution from step c) into the module through the lumen of the hollow fibers to allow an interfacial polymerization reaction to take place, and
h) rinsing the module with an aqueous solvent by injection through a module inlet.
11. A porous hollow fiber membrane modified with a thin film composite (TFC) layer comprising aquaporin water channels, wherein the TFC layer is a polyamide layer provided on the inside of the fibers.
12. The HF membrane according to claim 11 , wherein said aquaporin water channels are selected from the group consisting of AqpZ channels and SoPIP2;1 water channels.
13. The HF module of claim 11 , wherein said aquaporin water channels are incorporated in vesicles before incorporation into the TFC layer.
14. The HF module of claim 13 , wherein said vesicles into which the aquaporin water channels are incorporated are liposomes or polymersomes.
15. The HF module of claim 14 , wherein said liposomes are prepared from lipids selected from the group consisting of DPhPC, DOPC, mixed soy bean lipids, asolectin and E. coli mixed lipids.
16. The HF module of claim 15 , wherein said polymersomes comprise triblock copolymers of the hydrophile-hydrophobe-hydrophile (A-B-A or A-B-C) type, diblock copolymers of the hydrophile-hydrophobe type (A-B), or a combination thereof.
17. The HF membrane according to claim 13 , wherein said TFC layer is formed through interfacial polymerization of an aqueous solution of a di- or triamine with a solution of a di- or triacyl halide in an organic solvent, and wherein the aquaporin water channel vesicles are incorporated in said aqueous solution.
18. The HF module of claim 11 , wherein the fibers are polyethersulfone fibers.
19. The method of claim 10 , wherein the fibers are polyethersulfone fibers.
20. The method according to claim 10 , wherein the method comprises the steps of
a) obtaining an aquaporin vesicles suspension, wherein said vesicles are proteoliposomes or proteopolymersomes having from about 25 to about 500 LPR/POPR of protein,
b) preparing an aqueous solution of 1,3-diaminobenzene of about 1% to about 5% (w/w) concentration,
c) dissolving benzene-1,3,5-tricarbonyl chloride in an organic solvent selected from the group consisting of hexane, heptane, octane or a mixture of solvents to obtain a concentration of about 0.05% to about 1% (w/v),
d) preparing an 1,3-diaminobenzene/aquaporin vesicle mixture by dissolving/mixing the vesicles preparation from step a) with the solution from step b),
e) pumping the mixture from step d) through the lumen of the hollow fibers in a hollow fiber module using its end inlet,
f) removing excess aqueous solution by a gas purging of the lumen side of the fibers using a module inlet,
g) injecting the benzene-1,3,5-tricarbonyl chloride solution from step c) into the module through the lumen of the hollow fibers to allow an interfacial polymerization reaction to take place, and
h) rinsing the module with an aqueous solvent by injection through a module inlet.
21. The method according to claim 10 , wherein in step f) the fibers are gas purged to dryness and wherein the module is held upside down in step f).