Process to control CO concentrations in fermentations
View Patent ↗A process for controlling concentration of CO in a bioreactor provides a direct and real time measurement of dissolved CO in a fermentation medium. The process for controlling concentrations of CO in a bioreactor includes contacting an aliquot of fermentation medium with at least one CO binding ligand and at least one microbial inactivator.
1. A process for measuring a concentration of CO in an aqueous fermentation medium, the process comprising:
i) contacting an aliquot of the fermentation medium that includes acetogenic bacteria with at least one CO binding ligand and at least one microbial inactivator selected from the group consisting of a salt, acid, base, organic solvent, oxidizing agent, heat, cold and mixtures thereof, wherein the sat is selected from the group consisting of sodium chloride, potassium chloride ammonium chloride sodium bromide and mixtures thereof; and
ii) measuring an amount of CO bound to the CO binding ligand.
2. The process of claim 1 wherein the CO bonding ligand is selected from the group consisting of hemoglobin, myoglobin, free heme, heme containing compounds, and mixtures thereof.
3. The process of claim 1 wherein the amount of CO bound to the CO binding ligand is measured by determining changes in absorbance.
4. The process of claim 1 wherein the acetogenic bacterium is selected from the group consisting of Acetogenium kivui, Acetoanaerobium noterae, Acetobacterium woodii, Alkalibaculum bacchi CP11 (ATCC BAA-1772), Blautia producta, Butyribacterium methylotrophicum, Caldanaerobacter subterraneous, Caldanaerobacter subterraneous pacificus, Carboxydothermus hydrogenoformans, Clostridium aceticum, Clostridium acetobutylicum, Clostridium acetobutylicum P262 (DSM 19630 of DSMZ Germany), Clostridium autoethanogenum (DSM 19630 of DSMZ Germany), Clostridium autoethanogenum (DSM 10061 of DSMZ Germany), Clostridium autoethanogenum (DSM 23693 of DSMZ Germany), Clostridium autoethanogenum (DSM 24138 of DSMZ Germany), Clostridium carboxidivorans P7 (ATCC PTA-7827), Clostridium coskatii (ATCC PTA-10522), Clostridium drakei, Clostridium ljungdahlii PETC (ATCC 49587), Clostridium ljungdahlii ERI2 (ATCC 55380), Clostridium ljungdahlii C-01 (ATCC 55988), Clostridium ljungdahlii O-52 (ATCC 55889), Clostridium magnum, Clostridium pasteurianum (DSM 525 of DSMZ Germany), Clostridium ragsdali P11 (ATCC BAA-622), Clostridium scatologenes, Clostridium thermoaceticum, Clostridium ultunense, Desulfotomaculum kuznetsonii, Eubacterium limosum, Geobacter sulfurreducens, Methanosarcina acetivorans, Methanosarcina barkeri, Morrella thermoacetica, Morrella thermoautotrophica, Oxobacter pfennigii, Peptostreptococcus productus, Runtinococcus productus, Thermoanaerobacter kivui , and mixtures thereof.
5. The process of claim 1 wherein the microbial inactivator is a salt solution having a concentration of about 0.2 to about 5M.
6. The process of claim 1 wherein the microbial inactivator is an acid solution having a concentration of about 0.02 to about 5M.
7. The process of claim 1 wherein the microbial inactivator is a base solution having a concentration of about 0.02 to about 5M.