Methods for amplifying DNA from the promoter of the NDRG4 gene DNA obtained from a fecal sample
View Patent ↗A method of detecting a predisposition to, or the incidence of, cancer in a sample comprises detecting an epigenetic change in at least one gene selected from an NDRG4/NDRG2 subfamily gene, GATA4, OSMR, GATA5, SFRP1, ADAM23, JPH3, SFRP2, APC, MGMT, TFPI2, BNIP3, FOXE1, SYNE1, SOX17, PHACTR3 and JAM3, wherein detection of the epigenetic change is indicative of a predisposition to, or the incidence of, cancer. Also described are pharmacogenetic methods for determining suitable treatment regimens for cancer and methods for treating cancer patients, based around selection of the patients according to the methods of the invention. The present invention is also concerned with improved methods of collecting, processing and analyzing samples, in particular body fluid samples. These methods may be useful in diagnosing, staging or otherwise characterizing various diseases. The invention also relates to methods for identifying, diagnosing, staging or otherwise characterizing cancers, in particular gastrointestinal cancers such as colorectal cancers, gastric cancers and oesophageal cancers. The methods of the invention relate, inter alia, to isolating and analyzing the human DNA component from fecal samples and blood-based samples.
1. A method comprising:
(a) obtaining DNA from a fecal sample;
(b) treating the DNA obtained from the fecal sample with a reagent that converts non-methylated cytosines to uracil;
(c) amplifying the treated DNA with primers that hybridize to CpG dinucleotides in a target sequence that is within positions 1-1000 of SEQ ID NO:524 to produce amplified DNA that comprises CpG dinucleotides; and
(d) detecting production of the amplified DNA using a probe that hybridizes to CpG dinucleotides in the amplified DNA by detecting hybridization of the probe to the amplified DNA.
2. A method comprising:
(a) treating DNA obtained from a fecal sample with a reagent that converts non-methylated cytosines to uracil;
(b) amplifying the treated DNA with primers that hybridize to CpG dinucleotides in a target sequence that is within positions 1-1000 of SEQ ID NO:524 to produce amplified DNA that comprises CpG dinucleotides
(c) detecting the production of amplified DNA using a probe that hybridizes to CpG dinucleotides in the amplified DNA by detecting hybridization of the probe to the amplified DNA.
3. The method of claim 1 ,
wherein the probe is a hydrolytic probe and hybridization of the probe to the amplified DNA is detected by detecting hydrolysis of the probe.
4. The method of claim 2 , wherein the probe is a hydrolytic probe and hybridization of the probe to the amplified DNA is detected by detecting hydrolysis of the probe.