Biological Sample Preparation Devices And Methods
A device according to various embodiments can include a first chamber and a second chamber configured to contain at least one biological sample. A triturating element is interdisposed between the first chamber and the second chamber and provides fluid communication between the first chamber and the second chamber.
1 . A device for preparing a biological sample for analysis, the device comprising:
a first chamber configured to contain at least one biological sample;
a second chamber configured to contain at least one biological sample; and
a triturating element interdisposed between the first chamber and the second chamber and providing fluid communication between the first chamber and the second chamber.
2 . The device of claim 1 , wherein the first chamber, the second chamber, and the triturating element are at least partially defined by a common surface.
3 . The device of claim 1 , wherein the first and second chambers are collapsible and configured such that alternate collapsing of the first chamber and the second chamber flows the at least one biological sample through the triturating element to facilitate sample preparation.
4 . The device of claim 1 , further comprising an outlet port configured to flow a prepared biological sample into at least one reaction zone.
5 . The device of claim 4 , wherein the first and second chambers are collapsible and wherein collapsing of at least one of the first and second chambers transfers the prepared biological sample through the outlet port.
6 . The device of claim 5 , wherein the first and second chambers are configured such that simultaneous collapsing of the first and second chambers transfers the prepared biological sample through the outlet port.
7 . The device of claim 1 , wherein the triturating element is configured to disrupt at least one cell of the at least one biological sample.
8 . The device of claim 1 , wherein the triturating element defines at least one through-hole.
9 . The device of claim 8 , wherein the at least one through-hole has a circular cross- section.
10 . The device of claim 8 , further comprising at least one geometric structure disposed within the at least one through-hole.
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15 . The device of claim 8 , wherein the triturating element defines a plurality of through-holes.
16 . The device of claim 15 , wherein at least some of the plurality of through-holes have differing configurations.
17 . The device of claim 1 , wherein a configuration of the triturating element is selected based upon a shearing rate.
18 . The device of claim 17 , wherein the shearing rate is selected based upon at least one cell of the at least one biological sample selected for disrupting.
19 . The device of claim 1 , wherein the first and second chambers comprise fluidic bags.
20 . The device of claim 1 , wherein at least one of the first and second chambers is configured to be preloaded with at least one material for facilitating sample preparation.
21 . The device of claim 1 , wherein at least one of the first and second chambers is collapsible.
22 . The device of claim 1 , wherein the triturating element is configured to disrupt at least some cells contained in the at least one biological sample.
23 . The device of claim 1 , wherein the device comprises a microfluidic device.
24 . The device of claim 1 , wherein the device is disposable.
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