Shaped fiber-based products and method of manufacture thereof
View Patent ↗The present invention relates to shaped, bone fiber-based products and methods to make the same.
1. A method of forming a shaped, bone fiber-based product of specific dimensions comprising:
cutting bone into fibers;
at least partially demineralizing the fibers, wherein the demineralized fibers comprise at least one growth factor, wherein the at least one growth factor is IGF-1, BMP-2, BMP-4 or BMP-7;
entangling the demineralized fibers in a solvent to form entangled demineralized bone fibers;
placing the entangled demineralized bone fibers into a mold; and
drying the entangled demineralized bone fibers in the mold while warming under reduced pressure.
2. The method of claim 1 , wherein the product is a shape selected from the group consisting of a cube, a block, and a strip.
3. The method of claim 1 , wherein the bone is selected from the group consisting of cortical bone, cancellous bone, and combinations thereof.
4. The method of claim 1 , wherein the drying step heats the mold to a temperature between about 30° C. to about 80° C.
5. The method of claim 1 , wherein the drying is lyophilization.
6. The method of claim 1 , wherein the drying is heating the mold under reduced pressure of between about 1 nTorr to about 740 Torr.
7. The method of claim 1 , further comprising entangling the demineralized fibers with at least one of a wood, a food stuff, a plant-based material, an elastomer, or a thermoplastic.
8. The method of claim 1 , wherein a void to fiber ratio of the product is between about 1:99 and about 1:1.
9. The method of claim 1 , wherein a length of the fibers is between about 1 mm and about 200 mm.
10. The method of claim 1 , wherein an average width or diameter of the fibers is between about 0.1 mm and about 30 mm.
11. The method of claim 1 , wherein a thickness of the fibers is between about 0.01 mm and about 1 mm.
12. The method of claim 1 , wherein the step of cutting bone into fibers comprises using a cutting blade at an angle of between about 30° and about 90° from the native grain of the bone.
13. The method of claim 1 , wherein the solvent is selected from the group consisting of an aqueous acid, an aqueous base, a balanced salt solution, and a buffer.
14. The method of claim 1 , wherein the product has a residual moisture content of less than about 6%.
15. The method of claim 1 , wherein the step of drying the entangled fibers is conducted for between about 1 hour and about 48 hours.
16. The method of claim 1 , further comprising rehydrating the product to between about 60% and about 100% of a property selected from the group consisting of an initial shape, flexibility or compressibility.
17. The method of claim 1 , wherein the solvent is a water miscible, low boiling solvent.
18. The method of claim 1 , wherein a ratio of the solvent to the demineralized fibers is between about 5:95 and about 95:1.
19. The method of claim 1 , wherein the step of drying is conducted in a drying chamber and further comprises blowing gas through the drying chamber.
20. The method of claim 1 , wherein the bone is not demineralized at the time the bone is cut into fibers.
21. A method of forming a shaped, bone fiber-based product of specific dimensions comprising:
cutting bone into fibers;
at least partially demineralizing the fibers to form at least partially demineralized fibers, wherein the at least partially demineralized fibers comprise at least one growth factors, and wherein the at least partially demineralized fibers have a calcium content of less than about 8%, and wherein the growth factor is selected from the group consisting of IGF-1 in an amount greater than 60 pg of IGF-1 per gram of demineralized bone matrix, BMP-2 in an amount greater than 2700 pg of BMP-2 per gram of demineralized bone matrix, BMP-4 in an amount greater than 200 pg of BMP-4 per gram of demineralized, BMP-7 in an amount greater than 9000 pg of BMP-7 per gram of demineralized bone matrix, and combinations thereof;
entangling the partially demineralized fibers in a water miscible solvent to form entangled demineralized bone fibers, wherein a ratio of the water miscible solvent volume to a mass of the demineralized bone fibers is between about 0.5 mL of solvent/gram of fibers and about 30 mL solvent/gram of fibers;
placing the entangled demineralized bone fibers into a mold; and
drying the entangled demineralized bone fibers in the mold while warming under reduced pressure.
22. The method of claim 21 , wherein the solvent is selected from the group consisting of an acetone, an acetonitrile, an ethanol, a methanol, a tetrahydrofuran, and combinations thereof.