Sulfonylurea-responsive repressor proteins
Compositions and methods relating to the use of sulfonylurea-responsive repressors are provided. Compositions include polypeptides that specifically bind to an operator, wherein the specific binding is regulated by a sulfonylurea compound. Compositions also include polynucleotides encoding the polypeptides as well as constructs, vectors, prokaryotic and eukaryotic cells, and eukaryotic organisms including plants and seeds comprising the polynucleotide, and/or produced by the methods. Also provided are methods to provide a sulfonylurea-responsive repressor to a cell or organism, and to regulate expression of a polynucleotide of interest in a cell or organism, including a plant or plant cell.
1. A method of regulating transcription of a polynucleotide of interest in a host cell comprising:
(a) providing the host cell comprising the polynucleotide of interest, wherein the polynucleotide of interest is operably linked to a promoter comprising at least one operator sequence;
(b) providing a recombinant sulfonylurea-responsive repressor polypeptide that specifically binds to the operator sequence; and,
(c) providing a sulfonylurea compound, wherein the sulfonylurea binds to the polypeptide to form a complex that modifies the binding properties of the polypeptide to the operator,
wherein the sulfonylurea-responsive polypeptide comprises an amino acid sequence having at least 95% sequence identity when compared to the full-length sequence set forth in SEQ ID NO:1233.
2. The method of claim 1 , wherein the host cell is a eukaryotic cell.
3. The method of claim 2 , wherein the eukaryotic cell is a plant cell.
4. The method of claim 3 , wherein the plant cell is from soybean, rice, corn, tobacco, sorghum, sugarcane, barley, oat, wheat, turf grass, canola, cotton, sunflower, safflower, or alfalfa.
5. The method of claim 1 , wherein providing the polypeptide comprises contacting the cell with an expression cassette comprising a promoter functional in the cell operably linked to a polynucleotide that encodes the polypeptide.
6. The method of claim 1 , wherein the plant cell is contained in a plant or a seed.
7. The method of claim 1 , wherein the plant cell is from a monocot or a dicot.