Compositions and methods for topical diagnostic and therapeutic transport
Compositions and methods are provided that are useful for the delivery, including transdermal delivery, of biologically active agents, such as non-protein non-nucleotide therapeutics and protein-based therapeutics excluding insulin, botulinum toxins, antibody fragments, and VEGF. The compositions and methods are particularly useful for topical delivery of antifungal agents and antigenic agents suitable for immunization. Alternatively, the composition can be prepared with components useful for targeting the delivery of the compositions as well as imaging components.
1. A method of administering a non-protein, non-nucleotide biologically active agent to a subject in need thereof, said method comprising:
topically applying to the skin or epithelium of the subject:
the non-protein, non-nucleotide biologically active agent;
a positively charged carrier, the carrier comprising:
a polypeptide or a polyalkyleneimine backbone having attached thereto:
one or more positively charged branching groups selected from the group consisting of:
-(gly) n1 -(arg) n2 (SEQ ID NO. 1),
(gly) p -RGRDDRRQRRR-(gly) q (SEQ ID NO. 2),
(gly) p -YGRKKRRQRRR-(gly) q (SEQ ID NO. 3),
(gly) p -RKKRRQRRR-(gly) q (SEQ ID NO. 4), and
Antennapedia protein transduction domain (PTD) peptide;
wherein the subscript n1 is an integer of from 0 to about 20, and the subscript n2 is independently an odd integer of from about 5 to about 25; and the subscripts p and q are each independently an integer of from 0 to 20,
and wherein the carrier is applied in an effective amount to provide for transdermal delivery of the biologically active agent;
wherein the association between the carrier and the biologically active agent is direct and non-covalent and without a negatively charged backbone; and
wherein the biologically active agent and the carrier form a non-covalent complex wherein the carrier is the sole necessary agent for transdermal delivery of the biologically active agent.
2. The method according to claim 1 wherein the composition provides greater transdermal delivery of the biologically active agent relative to the agent in the absence of the carrier.
3. The method according to claim 2 in which the biologically active agent has a therapeutic activity.
4. The method according to claim 1 in which the biologically active agent and carrier are administered to the subject in a composition containing both components.
5. The method according to claim 1 in which the biologically active agent and carrier are administered separately to the subject.
6. The method according to claim 3 in which the biologically active agent and carrier are administered to the subject in a composition containing both components.
7. The method according to claim 3 in which the biologically active agent and carrier are administered separately to the subject.
8. The method according to claim 1 in which the composition is a controlled release composition.
9. The method according to claim 3 in which the composition is a controlled release composition.
10. The method according to claim 1 in which the biologically active agent is an antifungal agent.
11. The method according to claim 1 in which the biologically active agent is an agent for treating or preventing symptoms of psoriasis.
12. The method according to claim 10 in which an antifungal agent and carrier are administered to the subject in a composition containing both components.
13. The method according to claim 10 in which the antifungal agent and carrier are administered separately to the subject.
14. The method according to claim 10 in which the composition is a controlled release composition.
15. The method according to claim 10 in which the antifungal agent is selected from amphotericin B, fluconazole, flucytosine, itraconazole, ketoconazole, clotrimazole, econozole, griseofulvin, miconazole, nystatin, ciclopirox and the like.
16. The method according to claim 10 in which the antifungal agent is administered to treat the symptoms and signs of a fungal infection.
17. The method according to claim 10 in which the antifungal agent is administered to alter symptoms or signs of fungal infection of the nail plate or nail bed.