Acceptor framework for CDR grafting
View Patent ↗The present invention relates to an antibody acceptor framework and to methods for grafting non-human antibodies, e.g., rabbit antibodies, using a particularly well suited antibody acceptor framework. Antibodies generated by the methods of the invention are useful in a variety of diagnostic and therapeutic applications.
1. An isolated nucleic acid encoding a human heavy chain acceptor framework comprising SEQ ID NO: 1, wherein the human heavy chain acceptor framework comprises an amino acid substitution at position 12, 103, and/or 144 (Aho numbering).
2. The isolated nucleic acid of claim 1 , wherein the substitution is
(a) Serine (S) at position 12;
(b) Serine (S) or Threonine (T) at position 103; and/or
(c) Serine (S) or Threonine (T) at position 144.
3. An isolated nucleic acid encoding a human heavy chain acceptor framework comprising SEQ ID NO: 1.
4. A vector comprising the nucleic acid of claim 3 .
5. A host cell comprising the vector of claim 4 .