HARVESTING ALGAE FROM WATER
The present application includes methods to harvest a non-vascular photosynthetic organism (NVPO) such as microalgae from an aqueous culture comprising brackish, non-brackish, marine, sea or saline water using polymer flocculants. The methods are suitable for harvesting NVPO from aqueous culture with total dissovled solids (TDS) of at least 1500 mg/L. Methods are also provided to harvest a NVPO using flocculation with or without a Dissolved Air Flotation (DAF) process. Methods are further provided to flocculate and harvest a NVPO directly in a pond. The present application further provides NVPO-containing intermediates, compositions, or products produced by the methods provided herein.
1 - 81 . (canceled)
82 . A method to harvest a non-vascular photosynthetic organism (NVPO) from an aqueous culture, comprising, mixing an effective amount of a cationic organic polymer flocculant with said aqueous culture of said NVPO to form floes of said NVPO; and collecting said flocs of said NVPO, wherein said aqueous culture has a total dissolved solids (TDS) of at least 1500 milligram per liter (mg/L).
83 . The method of claim 82 , wherein said floes are collected by sedimentation.
84 . The method of claim 82 , further comprising, introducing dissolved air into said mixed aqueous culture; and collecting said flocs by dissolved air floatation.
85 . The method of claim 82 , wherein said aqueous culture is an open pond culture.
86 . The method of claim 82 , wherein said aqueous culture comprises a TDS selected from the group consisting of between 1500 and 35000, between 5000 and 35000, and between 15000 and 35000 mg/L.
87 . The method of claim 82 , wherein said NVPO in said aqueous culture is at a concentration between 0.001% and 0.2%, and said effective amount of said cationic organic polymer flocculant is less than 10 parts per million (ppm).
88 . The method of claim 82 , wherein said method produces a biomass slurry comprising said NVPO and said cationic organic polymer flocculant, said NVPO in said harvested biomass slurry is at a concentration of at least 0.2%, and the ratio between the weight of said cationic organic polymer flocculant and the Ash-Free Dry Weight (AFDW) of said NVPO in said harvested biomass slurry is at most 1.5%.
89 . The method of claim 82 , wherein said method produces a aqueous medium comprising said NV PO at a concentration between 0.0001% and 0.02%, and said cationic organic polymer flocculant at a concentration of at most 1 parts per million (ppm).
90 . The method of claim 82 , wherein said cationic organic polymer flocculant has a stock concentration selected from the group consisting of 0.1%, 0.25%, 0.5%, 0.75%, and 1%.
91 . The method of claim 82 , wherein said cationic organic polymer flocculant has no significant impacts on the group consisting of growth of said NVPO, pH of said aqueous culture, nitrate level of said aqueous culture, and phosphate level of said aqueous culture.
92 . The method of claim 82 , wherein said flocs of said NVPO have an average diameter selected from the group consisting of at least 0.05, 0.1, 0.2, 0.3, 0.5, 1, 2, 5, 10, 20, 30, 40, 50, and 60 millimeters.
93 . The method of claim 82 , wherein at least 90% of said NVPO in said aqueous culture is harvested through said method.
94 . The method of claim 82 , wherein said method achieves a harvest volume of less than 30% of the volume of said aqueous solution.
95 . The method of claim 82 , wherein said NVPO is from a genus selected from the group consisting of Chlamydomonas, Nannochloropsis, Scenedesmus, Desmodesmus, Tetraselmis , and Arthrospira.
96 . The method of claim 82 , wherein said method further comprises mixing an effective amount of an anionic polymer flocculant with said aqueous culture of said NVPO.
97 . A method to harvest a non-vascular photosynthetic organism (NVPO) from an open pond aqueous culture by dissolved air flotation, comprising mixing an effective amount of a cationic organic polymer flocculant with said open pond aqueous culture to form flocs of said NVPO; introducing dissolved air into said mixed aqueous culture; and collecting said floes of said NVPO, wherein said aqueous culture has a total dissolved solids (TDS) of at least 1500 milligram per liter (mg/L).
98 . The method of claim 97 , wherein said aqueous culture comprises a TDS selected from the group consisting of between 1500 and 35000, between 5000 and 35000, and between 15000 and 35000 mg/L.
99 . The method of claim 97 , wherein said NVPO in said aqueous culture is at a concentration between 0.001% and 0.2%, and said effective amount of said polymer flocculant is less than 10 parts per million (ppm).
100 . The method of claim 97 , wherein said polymer flocculant has no significant impacts on the group consisting of growth of said NVPO, pH of said aqueous culture, nitrate level of said aqueous culture, and phosphate level of said aqueous culture.
101 . The method of claim 97 , wherein said NVPO is from a genus selected from the group consisting of Nannochloropsis, Scenedesmus, Desmodesmus, Tetraselmis , and Arthrospira.