Microfluidic device
View Patent ↗A microfluidic device includes a first substrate made of a first polymer material and a second substrate made of a second polymer material, the first and second substrates having respective bonding surfaces, at least one of the bonding surfaces having channel formations so that, when the bonding surfaces are bonded by surface deformation to one another, the bonded first and second substrates and the channel formations form at least part of a microfluidic channel network comprising a plurality of microfluidic channels, wherein one or more indicator pits, separate to the channel formations defining the microfluidic channel network, are formed in at least one of the bonding surfaces, so that surface deformation caused by the bonding process causes a change of configuration of the one or more indicator pits.
1. A microfluidic device comprising:
a first substrate made of a first polymer material and a second substrate made of a second polymer material, the first and second substrates having respective bonding surfaces, at least one of the bonding surfaces having channel formations so that, when the bonding surfaces are bonded by surface deformation to one another, the bonded first and second substrates and the channel formations form at least part of a microfluidic channel network comprising a plurality of microfluidic channels,
wherein one or more indicator pits, separate to the channel formations defining the microfluidic channel network, are formed in at least one of the bonding surfaces, so that surface deformation caused by the bonding process causes a change of configuration of the one or more indicator pits,
at least one pair of pits is provided at corresponding positions on the bonding surfaces of each of the first and second substrates so as to provide an alignment indicator for assembly of the microfluidic device.
2. The device of claim 1 , wherein the indicator pits are arranged adjacent to the microfluidic channels.
3. The device of claim 1 , wherein one or more indicator pits are arranged adjacent to features of the microfluidic channels including at least one of: a junction between microfluidic channels; an inlet of a microfluidic channel from a port, reservoir or chamber; an outlet of a microfluidic channel from a port, reservoir or chamber; a bend in a microfluidic channel; and a portion of a microfluidic channel where an electrode is arranged.
4. The device of claim 1 , wherein the indicator pits are fluidically isolated from the microfluidic channels.
5. The device of claim 1 , wherein the indicator pits have a plurality of levels or a varying level between a minimum indicator pit depth and a maximum indicator pit depth, each of the plurality of levels being formed by a plateau that lies parallel to the upper and lower surfaces.
6. The device of claim 1 , wherein the microfluidic channels have a channel depth and indicator pits have a maximum indicator pit depth less than the channel depth.
7. The device of claim 1 , wherein the indicator pits are formed in that one of the first and second substrates which has the majority of the structures defining the microfluidic channels.
8. The device of claim 1 , comprising one or more groups of adjacent indicator pits, each indicator pit in the group having a different respective depth.
9. The device of claim 1 , in which the substrates are flat.
10. The device of claim 1 , comprising one or more complementary inter-engaging formations on the respective bonding surfaces.