IP Library Granted Patent US 10,118,918
Granted Patent B2
US 10,118,918 · App. 14/654,161 · Granted Nov 6, 2018

PRMT5 inhibitors and uses thereof

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Quick Facts
Patent No.
US 10,118,918
App. No.
14/654,161
Granted
Nov 6, 2018
Kind
B2
Abstract

Described herein are compounds of formula (A), pharmaceutically acceptable salts thereof, and pharmaceutical compositions thereof. Compounds of the present invention are useful for inhibiting PRMT5 activity. Methods of using the compounds for treating PRMT5-mediated disorders are also described.

Claims (64)

1. A compound of Formula (A):

or a pharmaceutically acceptable salt thereof;

wherein:

R 12 is hydrogen, and —R 13 is halogen; or

R 12 is hydrogen, and R 13 is —OR 1 ;

R 1 is hydrogen, R z , or —C(O)R z , wherein R z is optionally substituted C 1-6 alkyl;

L z is —C(O)N(R)— or —X A —C(R 2A )(R 3A )C(═O)N(R)—, wherein X A is —CR 4A R 5A —, and each instance of R 2A , R 3A , R 4A , and R 5A is hydrogen;

R is hydrogen or optionally substituted C 1-6 aliphatic;

Ring Z is a monocyclic or bicyclic aromatic ring having 0-4 heteroatoms selected from nitrogen, wherein Ring Z is substituted with 0, 1, 2, 3, 4, or 5 R y groups, as valency permits;

each R y is independently selected from the group consisting of halo, —CN, —NO 2 , optionally substituted aliphatic, optionally substituted carbocyclyl, optionally substituted aryl, optionally substituted heterocyclyl, optionally substituted heteroaryl, —OR A , —N(R B ) 2 , —SR A , —C(═O)R A , —C(O)OR A , —C(O)SR A , —C(O)N(R B ) 2 , —C(O)N(R B )N(R B ) 2 , —OC(O)R A , —OC(O)N(R B ) 2 , —NR B C(O)R A , —NR B C(O)N(R B ) 2 , —NR B C(O)N(R B )N(R B ) 2 , —NR B C(O)OR A , —SC(O)R A , —C(═NR B )R A , —C(═NNR B )R A , —C(═NOR A )R A , —C(═NR B )N(R B ) 2 , —NR B C(═NR B )R B , —C(═S)R A , —C(═S)N(R B ) 2 , —NR B C(═S)R A , —S(O)R A , —OS(O) 2 R A , —SO 2 R A , —NR B SO 2 R A , and —SO 2 N(R B ) 2 ;

each R A is independently selected from the group consisting of hydrogen, optionally substituted aliphatic, optionally substituted carbocyclyl, optionally substituted heterocyclyl, optionally substituted aryl, and optionally substituted heteroaryl;

each R B is independently selected from the group consisting of hydrogen, optionally substituted aliphatic, optionally substituted carbocyclyl, optionally substituted heterocyclyl, optionally substituted aryl, and optionally substituted heteroaryl, or two R B groups are taken together with their intervening atoms to form an optionally substituted heterocyclic ring;

R 21 , R 22 , R 23 , and R 24 are each independently hydrogen, halo, or optionally substituted aliphatic;

each R x is independently selected from the group consisting of halo, —CN, optionally substituted aliphatic, and —OR′;

R′ is hydrogen or optionally substituted aliphatic;

n is 0, 1, 2, 3, 4, 5, 6, 7, or 8; and

x is 1, and y is 1; or

x is 0, and y is 2, 3, or 4; or

x is 1, and y is 3.

2. The compound of claim 1 , wherein the compound is of Formula (A-3-iv):

or a pharmaceutically acceptable salt thereof, wherein R 12 is hydrogen.

3. The compound of claim 1 , wherein Ring Z is of the formula:

X 1 , X 2 , X 3 , and X 4 are independently selected from the group consisting of N, CH, and CR y , provided that at least one of X 2 , X 3 , and X 4 is not N;

L 1 is a bond, —O—, or —N(R)—;

R is hydrogen or optionally substituted C 1-6 aliphatic; and

Cy D is an optionally substituted 5- to 6-membered heteroaryl having 1-3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, or an optionally substituted 3- to 8-membered heterocyclic ring having 1-2 heteroatoms independently selected from nitrogen, oxygen, and sulfur.

4. The compound of claim 3 , wherein:

X 1 , X 2 , X 3 , and X 4 are independently selected from the group consisting of CH and CR y ; or

X 1 is N, and each of X 2 , X 3 , and X 4 are independently selected from the group consisting of CH and CR y ; or

X 2 is N, and each of X 1 , X 3 , and X 4 are independently selected from the group consisting of CH and CR y ; or

X 3 is N, and each of X 1 , X 2 , and X 4 are independently selected from the group consisting of CH and CR y ; or

X 4 is N, and each of X 1 , X 2 , and X 3 are independently selected from the group consisting of CH and CR y ; or

each of X 1 and X 2 is N, and each of X 3 and X 4 is independently CH or CR y ; or

each of X 1 and X 3 is N, and each of X 2 and X 4 is independently CH or CR y ; or

each of X 1 and X 4 is N, and each of X 2 and X 3 is independently CH or CR y ; or

each of X 2 and X 4 is N, and each of X 1 and X 3 is independently CH or CR y ; or

each of X 2 and X 3 is N, and each of X 1 and X 4 is independently CH or CR y ; or

each of X 3 and X 4 is N, and each of X 1 and X 2 is independently CH or CR y .

5. The compound of claim 3 , wherein L 1 is —O—, or —N(R)—; R is hydrogen or optionally substituted C 1-6 aliphatic; and Cy D is an optionally substituted 3- to 8-membered heterocyclic ring having 1-2 heteroatoms independently selected from nitrogen, oxygen, and sulfur.

6. The compound of claim 3 , wherein the compound is of Formula (V D -c):

or a pharmaceutically acceptable salt thereof, wherein L D is —C(O)N(R)—.

7. The compound of claim 1 , wherein L z is —C(O)N(R)—.

8. The compound of claim 1 , wherein L z is —X A —C(R 2A )(R 3A )C(═O)N(R)—.

9. The compound of claim 1 , wherein R 1 is hydrogen.

10. The compound of claim 1 , wherein n is 0.

11. The compound of claim 1 , wherein Ring Z is a monocyclic aromatic ring having 0-4 heteroatoms selected from nitrogen and wherein Ring Z is substituted with 1 or 2 R y groups, as valency permits;

R y is independently selected from the group consisting of optionally substituted carbocyclyl, optionally substituted aryl, optionally substituted heterocyclyl, optionally substituted heteroaryl, —OR A , and —N(R B ) 2 ;

each R A is independently selected from the group consisting of optionally substituted carbocyclyl, optionally substituted heterocyclyl, optionally substituted aryl, and optionally substituted heteroaryl; and

each R B is independently selected from the group consisting of hydrogen, optionally substituted carbocyclyl, optionally substituted heterocyclyl, optionally substituted aryl, and optionally substituted heteroaryl, or two R B groups are taken together with their intervening atoms to form an optionally substituted heterocyclic ring.

12. The compound of claim 1 , wherein Ring Z is selected from the group consisting of:

13. The compound of claim 1 selected from the group consisting of:

and pharmaceutically acceptable salts thereof.

14. The compound of claim 1 selected from the group consisting of:

and pharmaceutically acceptable salts thereof.

15. The compound of claim 1 selected from the group consisting of:

and pharmaceutically acceptable salts thereof.

16. A pharmaceutical composition comprising a compound of claim 1 , or a pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable excipient.

17. A kit or packaged pharmaceutical comprising a compound of claim 1 , or a pharmaceutically acceptable salt thereof, and instructions for use thereof.

18. A pharmaceutical composition comprising a compound of claim 13 , or a pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable excipient.

19. A kit or packaged pharmaceutical comprising a compound of claim 13 , or a pharmaceutically acceptable salt thereof, and instructions for use thereof.

20. A pharmaceutical composition comprising a compound of claim 14 , or a pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable excipient.

21. A kit or packaged pharmaceutical comprising a compound of claim 14 , or a pharmaceutically acceptable salt thereof, and instructions for use thereof.

22. A pharmaceutical composition comprising a compound of claim 15 , or a pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable excipient.

23. A kit or packaged pharmaceutical comprising a compound of claim 15 , or a pharmaceutically acceptable salt thereof, and instructions for use thereof.

Assignments (3)
TERMINATION AND RELEASE OF SECURITY INTEREST IN PATENTS AT REEL/FRAME: 051057/0848 Recorded Aug 13, 2022
From: BIOPHARMA CREDIT PLC
To: EPIZYME, INC.
Reel/Frame 061165/0501 →
SECURITY INTEREST Recorded Nov 19, 2019
From: EPIZYME, INC.
To: BIOPHARMA CREDIT PLC
Reel/Frame 051057/0848 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jun 26, 2015
From: DUNCAN, KENNETH W.; CHESWORTH, RICHARD; BORIACK-SJODIN, PAULA ANN; MUNCHHOF, MICHAEL JOHN
To: EPIZYME, INC.
Reel/Frame 035967/0395 →