ENHANCED PRODUCTION OF FATTY ACID DERIVATIVES
Genetically engineered cells and microorganisms are provided that produce fatty alcohols from the fatty acid biosynthetic pathway, as well as methods of their use.
1 - 18 . (canceled)
19 . A genetically modified bacterial cell for production of a fatty alcohol, comprising an exogenous nucleic acid sequence encoding a fatty acid derivative enzyme comprising an acyl-CoA reductase, wherein said bacterial cell converts a carbon source into a fatty alcohol.
20 . The bacterial cell of claim 19 , wherein said acyl-CoA reductase is encoded by a gene selected from the group consisting of acr1, acrM, BmFAR, mFAR1, and mFAR2.
21 . The bacterial cell of claim 20 , wherein said acyl-CoA reductase is encoded by said acr1 gene from Acinetobacter baylyi ADP1.
22 . The bacterial cell of claim 20 , wherein said acyl-CoA reductase is encoded by said acrM gene from Acinetobacter sp. M1.
23 . The bacterial cell of claim 20 , wherein said acyl-CoA reductase is encoded by said BmFAR gene from Bombyx mori.
24 . The bacterial cell of claim 20 , wherein said acyl-CoA reductase is encoded by said mFAR1 gene from Mus musculus.
25 . The bacterial cell of claim 20 , wherein said acyl-CoA reductase is encoded by said mFAR2 gene from Mus musculus.
26 . The bacterial cell of claim 19 , wherein said cell is further genetically modified to express an exogenous nucleic acid sequence encoding an acyl-CoA synthase.
27 . The bacterial cell of claim 26 , wherein said acyl-CoA synthase is encoded by a gene selected from the group consisting of fadD, fadDD35, yhfL, fadD1, fadD2, fadDD22, fadK, and faa3p.
28 . The bacterial cell of claim 27 , wherein said acyl-CoA synthase is encoded by said fadD gene from Escherichia coli.
29 . The bacterial cell of claim 27 , wherein said acyl-CoA synthase is encoded by said fadDD35 gene from Mycobacterium tuberculosis HR7Rv.
30 . The bacterial cell of claim 27 , wherein said acyl-CoA synthase is encoded by said yhfL gene from Bacillus subtilis.
31 . The bacterial cell of claim 27 , wherein said acyl-CoA synthase is encoded by said fadD1 gene from Pseudomonas aeruginosa.
32 . The bacterial cell of claim 27 , wherein said acyl-CoA synthase is encoded by said fadD2 gene from Pseudomonas aeruginosa PAO1.
33 . The bacterial cell of claim 27 , wherein said acyl-CoA synthase is encoded by said fadDD22 gene from Mycobacterium tuberculosis HR7Rv.
34 . The bacterial cell of claim 27 , wherein said acyl-CoA synthase is encoded by said fadK gene from Escherichia coli.
35 . The bacterial cell of claim 27 , wherein said acyl-CoA synthase is encoded by said faa3p gene from Saccharomyces cerevisiae.
36 . The bacterial cell of claim 26 , wherein said cell is further genetically modified to express an exogenous nucleic acid sequence encoding a thioesterase.
37 . The bacterial cell of claim 36 , wherein said thioesterase is encoded by a gene selected from the group consisting of tesA, ′tesA, tesB, fatB, fatB1, fatB2, fatB3, fatA, and fatA1.
38 . The bacterial cell of claim 37 , wherein said thioestrase is encoded by said tesA gene from Escherichia coli.
39 . The bacterial cell of claim 37 , wherein said thioestrase is encoded by said ′tesA gene from Escherichia coli.
40 . The bacterial cell of claim 37 , wherein said thioestrase is encoded by said tesB gene from Escherichia coli.
41 . The bacterial cell of claim 37 , wherein said thioestrase is encoded by said fatB gene from Cinnamonum camphorum, Umbellularia californica , or Arabidopsis thaliana.
42 . The bacterial cell of claim 37 , wherein said thioestrase is encoded by said fatB1 gene from Umbellularia californica.
43 . The bacterial cell of claim 37 , wherein said thioestrase is encoded by said fatB2 gene from Cuphea hookeriana.
44 . The bacterial cell of claim 37 , wherein said thioestrase is encoded by said fatB3 gene from Cuphea hookeriana.
45 . The bacterial cell of claim 37 , wherein said thioestrase is encoded by said fatA gene from Arabidopsis thaliana, Bradyrhiizobium japonicum , or Cuphea hookeriana.
46 . The bacterial cell of claim 37 , wherein said thioestrase is encoded by said fatA1 gene from Helianthus annus.
47 . The bacterial cell of claim 36 , wherein said cell is further genetically modified to express an exogenous nucleic acid sequence encoding an acetyl-CoA carboxylase.
48 . The bacterial cell of claim 47 , wherein said acetyl-CoA carboxylase is encoded by a gene selected from the group consisting of Escherichia coli, Desulfovibrio desulfuricans, Erwinia ( micrococcus ) amylovora, Kineococcus radiotolerans SRS30216 , Rhodospirillum rubrum , and Vibrio furnissii.
49 . The bacterial cell of claim 19 , wherein said bacterial cell is an Escherichia coli cell.
50 . The bacterial cell of claim 19 , wherein the fatty alcohol is released into a fermentation media.
51 . The bacterial cell of claim 50 , wherein the fermentation media comprises one or more of C8, C10, C12, C14, C16 and C18 fatty alcohols.
52 . The bacterial cell of claim 50 , wherein the fermentation media comprises unsaturated fatty alcohols.
53 . The bacterial cell of claim 50 , wherein the fermentation media comprises saturated fatty alcohols.
54 . The bacterial cell of claim 50 , wherein the fermentation media comprises straight chain fatty alcohols.
55 . The bacterial cell of claim 50 , wherein the fermentation media comprises branched chain fatty alcohols.
56 . A genetically modified bacterial cell for production of a fatty alcohol, comprising an exogenous nucleic acid sequence encoding a fatty acid derivative enzyme comprising an acyl-CoA reductase and an acyl-CoA synthase, wherein said bacterial cell converts a carbon source into a fatty alcohol.
57 . The bacterial cell of claim 56 , wherein said acyl-CoA reductase is encoded by an acr1 gene from Acinetobacter baylyi ADP1, and wherein said acyl-CoA synthase is encoded by a fadD gene from Escherichia coli.
58 . The bacterial cell of claim 56 , wherein said cell is further genetically modified to express an exogenous nucleic acid sequence encoding a thioesterase.
59 . The bacterial cell of claim 58 , wherein said thioesterase is encoded by a ′tesA gene from Escherichia coli.
60 . A genetically modified bacterial cell for production of a fatty alcohol, comprising an exogenous nucleic acid sequence encoding a fatty acid derivative enzyme comprising an acyl-CoA reductase, an acyl-CoA synthase, and a thioesterase, wherein said bacterial cell converts a carbon source into a fatty alcohol.
61 . The bacterial cell of claim 60 , wherein said acyl-CoA reductase is encoded by an acr1 gene from Acinetobacter baylyi ADP1, wherein said acyl-CoA synthase is encoded by a fadD gene from Escherichia coli , and wherein said thioesterase is encoded by a ′tesA gene from Escherichia coli.