Serpentine flow channels for flowing fluids over chip sensors
A nanopore based sequencing system is disclosed. The system includes a plurality of nanopore sensors, each nanopore sensor having a top portion for receiving a fluid. The system further includes an inlet delivering the fluid into the nanopore based sequencing system and an outlet delivering the fluid out of the nanopore based sequencing system. The system includes a fluid chamber that comprises one or more fluid flow channels above top portions of the nanopore sensors; wherein the fluid chamber includes at least one divider that limits the width of the one or more fluid flow channels. In some embodiments, the at least one divider limits the width of the one or more fluid flow channel based on whether the surface tension and adhesive forces between the fluid and the fluid flow channel surfaces are sufficient to prevent the fluid from collapsing within the fluid flow channel.
1. A nanopore based sequencing system, comprising:
a plurality of nanopore sensors, each nanopore sensor having a top portion for receiving a fluid;
an inlet delivering the fluid into the nanopore based sequencing system;
an outlet delivering the fluid out of the nanopore based sequencing system;
a fluid chamber that extends over top portions of the nanopore sensors;
a fan-out plenum adjacent to the inlet, providing a transition from the inlet to a first portion of the fluid chamber, wherein the first portion of the fluid chamber is wider than the inlet; and
a slit adjacent to the fan-out plenum, the slit draining the fluid from the fan-out plenum to the fluid chamber.
2. The nanopore based sequencing system of claim 1 , wherein the plurality of nanopore sensors are arranged into an array of sensor banks, wherein the array of sensor banks is arranged in rows and columns, and wherein the fluid chamber directs the fluid to flow above the array of sensor banks.
3. The nanopore based sequencing system of claim 2 , wherein the slit adjacent to the fan-out plenum spans across a width substantially the same as a row of the array of sensor banks.
4. The nanopore based sequencing system of claim 1 , further comprising:
a reverse fan-out plenum adjacent to the outlet, providing a transition from a second portion of the fluid chamber to the outlet, wherein the outlet is narrower than the second portion of the fluid chamber; and
a second slit adjacent to the reverse fan-out plenum, the second slit directing the fluid from the fluid chamber up the second slit to the reverse fan-out plenum.
5. The nanopore based sequencing system of claim 1 , wherein the fluid chamber comprises a curved roof.
6. The nanopore based sequencing system of claim 1 , wherein the fluid chamber comprises a D-shaped cross sectional geometry.
7. The nanopore based sequencing system of claim 1 , wherein a height of the fluid chamber is limited to one millimeter.
8. The method of forming a nanopore based sequencing system, comprising:
providing a plurality of nanopore sensors, each nanopore sensor having a top portion for receiving a fluid;
providing an inlet delivering the fluid into the nanopore based sequencing system;
providing an outlet delivering the fluid out of the nanopore based sequencing system;
providing a fluid chamber that extends over top portions of the nanopore sensors;
providing a fan-out plenum adjacent to the inlet, wherein the fan-out plenum provides a transition from the inlet to a first portion of the fluid chamber, wherein the first portion of the fluid chamber is wider than the inlet; and
providing a slit adjacent to the fan-out plenum, the slit draining the fluid from the fan-out plenum to the fluid chamber.
9. The method of claim 8 , wherein the plurality of nanopore sensors are arranged into an array of sensor banks, wherein the array of sensor banks is arranged in rows and columns, and wherein the fluid chamber directs the fluid to flow above the array of sensor banks.
10. The method of claim 9 , wherein the slit adjacent to the fan-out plenum spans across a width substantially the same as a row of the array of sensor banks.
11. The method of claim 8 , further comprising:
providing a reverse fan-out plenum adjacent to the outlet, wherein the reverse fan-out plenum provides a transition from a second portion of the fluid chamber to the outlet, wherein the outlet is narrower than the second portion of the fluid chamber; and
providing a second slit adjacent to the reverse fan-out plenum, the second slit directing the fluid from the fluid chamber up the second slit to the reverse fan-out plenum.
12. The method of claim 8 , wherein the fluid chamber comprises a curved roof.
13. The method of claim 8 , wherein the fluid chamber comprises a D-shaped cross sectional geometry.
14. The method of claim 8 , wherein a height of the fluid chamber is limited to one millimeter.