Detecting colorectal neoplasm

1. A method comprising:

extracting genomic DNA from a biological sample of a human individual suspected of having or having neoplasm, wherein the neoplasm is pre-cancerous, colorectal cancer, a large colorectal adenoma, or a sessile serrated polyp,

treating the extracted genomic DNA with bisulfite,

amplifying the bisulfite-treated genomic DNA using primers specific for three or less markers at least including CHST2 and VAV3, and

measuring methylation level of one or more CpG sites for CHST2 and VAV3 by methylation specific PCR, quantitative methylation specific PCR, methylation sensitive DNA restriction enzyme analysis, or bisulfite genomic sequencing PCR.

2. The method of claim 1 , wherein the biological sample is a stool sample, a tissue sample, a colorectal cyst sample, a colorectal tumor sample, a blood sample, or a urine sample.

3. The method of claim 1 ,

wherein the primers specific for VAV3 consist of SEQ ID NOS: 1 and 3, and

wherein the primers specific for CHST2 consist of SEQ ID NOS: 4 and 6.

4. The method of claim 1 , further comprising

amplifying the bisulfite-treated genomic DNA using primers specific for PDGFD, and

measuring methylation level of one or more CpG sites for PDGFD by methylation specific PCR, quantitative methylation specific PCR, methylation sensitive DNA restriction enzyme analysis, or bisulfite genomic sequencing PCR.

5. The method of claim 4 ,

wherein the primers specific for CHST2 consist of SEQ ID NOS: 16 and 18, and

wherein the primers specific for PDGFD consist of SEQ ID NOS: 55 and 57.