Porcine epidemic diarrhea virus vaccine
The present invention relates to a vaccine for protecting a pig against diseases associated with porcine epidemic diarrhea virus. The vaccine commonly includes inactivated/killed PEDV (e.g., chemically inactivated PED virus), and/or recombinant PEDV antigen and an adjuvant. Methods for protecting pigs against diseases associated with PEDV and methods of producing the porcine epidemic diarrhea virus vaccine are also provided.
1. An immunogenic composition comprising an inactivated whole porcine epidemic diarrhea virus (PEDV) and an oil-in-water emulsion as an adjuvant, wherein the porcine epidemic diarrhea virus (PEDV) is a PEDV of North American origin and that is encoded by SEQ ID NO:1, and/or comprises the sequence of SEQ ID NO:1, and/or comprises the RNA equivalent of SEQ ID NO:1, and/or that is encoded by SEQ ID NO:15.
2. The immunogenic composition of claim 1 , wherein the inactivated porcine epidemic diarrhea virus (PEDV) is chemically inactivated.
3. The immunogenic composition of claim 1 , wherein the inactivated porcine epidemic diarrhea virus (PEDV) is chemically inactivated by treatment with a chemical inactivating agent which includes a compound selected from the group consisting of ethylenimine, binary ethylenimine, acetylethylenimine and mixtures thereof.
4. The immunogenic composition of claim 1 , wherein the inactivated porcine epidemic diarrhea virus (PEDV) is chemically inactivated by treatment with binary ethylenimine.
5. The immunogenic composition of claim 1 , wherein the oil-in-water emulsion is an EMULSIGEN® oil-in-water emulsion-based adjuvant.
6. A kit for inducing an immunogenic response in a pig against porcine epidemic diarrhea virus (PEDV) comprising:
a. a dispenser capable of administering an immunogenic composition to a pig; and
b. the immunogenic composition according to claim 1 .
7. A method of producing a porcine epidemic diarrhea immunogenic composition comprising:
(a) inoculating simian cells with the porcine epidemic diarrhea virus (PEDV) composition of claim 1 ;
(b) incubating the inoculated simian cells;
(c) harvesting porcine epidemic diarrhea virus (PEDV) from the incubated cells; and
(d) treating the harvested cells with a chemical inactivating agent selected from the group consisting of ethylenimine, binary ethylenimine, acetylethylenimine or a mixture thereof to form an inactivated PEDV antigen.
8. The method of claim 7 , wherein the simian cells are Vero cells.
9. The method of claim 7 , wherein the chemical inactivating agent includes binary ethylenimine.
10. The method of claim 7 , further comprising adding an oil-in-water emulsion-based adjuvant EMULSIGEN® to the porcine epidemic diarrhea virus (PEDV) antigen.