Methods and compositions for obtaining useful plant traits
The present disclosure provides methods for obtaining plants that exhibit useful traits or that are useful for plant breeding by suppression of plastidic MSH1 in the presence of mitochondrial-targeted MSH1 in plants. Methods for identifying genetic loci that provide for useful traits in plants and plants produced with those loci are also provided. In addition, plants that exhibit the useful traits, parts of the plants including seeds, and products of the plants are provided as well as methods of using the plants. Recombinant DNA vectors and transgenic plants comprising those vectors that provide for suppression of plastidic MSH1 in the presence of mitochondrial-targeted MSH1 are also provided.
1. A plant or plant cell comprising a mutation in a MutS Homolog 1 (MSH1) gene polynucleotide sequence encoding the FYE amino acid sequence of the DNA binding domain 1 of the MSH1 polypeptide, wherein the mutation encodes the amino acid sequence FYZ in the DNA binding domain 1 wherein Z is any amino acid other than E, wherein such mutation is not present in control plants of the same species, wherein MSH1 function is suppressed in plastids of the plant or plant cell, and wherein MSH1 function is maintained in mitochondria of the plant or plant cell.
2. The plant or plant cell of claim 1 , wherein the mutation in the polynucleotide sequence that encodes the FYE amino acid sequence of the DNA binding domain 1 of MSH1 is a mutation that encodes the amino acid sequence FYA in the DNA binding domain.
3. The plant or plant cell of claim 1 , wherein the plant or plant cell is a Capsella rubella, Ricinus communis, Populus trichocarpa, Theobroma cacao, Citrus sinensis, Citrus clementine, Vitis vinifera, Prunus persica, Fragaria vesca subsp. Vesca, Solanum tuberosum, Cucumis sativus, Solanum lycopersicum, Cicer arietinum, Glycine max, Phaseolus vulgaris, Oryza sativa Japonica Group, Oryza sativa Indica Group, Oryza brachyantha, Setaria italica, Medicago truncatula, Zea mays, Cucumis sativus, Hordeum vulgare subsp. Vulgare, Sorghum bicolor, Aegilops tauschii , or Triticum aestivum plant or plant cell.