Mediator for test sensor
View Patent ↗A method of forming a 3-phenylimino-3H-phenothiazine or a 3-phenylimino-3H-phenoxazine mediator includes providing a first reactant including phenothiazine or phenoxazine, providing a first solvent, providing a second reactant and providing a second solvent. The first reactant, first solvent, second reactant and second solvent are combined to form a reactants solution. Sodium persulfate is added to the reactants solution to couple the first and second reactants resulting in a reaction solution including the 3-phenylimino-3H-phenothiazine or the 3-phenylimino-3H-phenoxazine mediator.
1. A composition including 3-phenylimino-3H-phenothiazine mediator formed by a process comprising the acts of:
providing a first reactant including phenothiazine;
providing a first solvent in which the phenothiazine has a desired solubility therein;
providing a second reactant to assist in forming the 3-phenylimino-3H-phenothiazine mediator;
providing a second solvent in which the second reactant has a desired solubility therein;
combining the first reactant, first solvent, second reactant and second solvent to form a third reactant solution;
adding sodium persulfate to the reactants solution to couple the first and second reactants resulting in a reaction solution including the 3-phenylimino-3H-phenothiazine mediator; and
after adding the sodium persulfate, further processing the third reactant solution including the 3-phenylimino-3H-phenothiazine mediator so as to isolate the 3-phenylimino-3H-phenothiazine mediator.
2. The composition of claim 1 , wherein the first solvent includes tetrahydrofuran (THF).
3. The composition of claim 1 , wherein the second solvent includes water.
4. The composition of claim 3 , wherein the second solvent further includes sodium hydroxide.
5. The composition of claim 1 wherein combining the first reactant, first solvent, second reactant and second solvent including the acts of combining the first reactant and the first solvent to form a first reactant solution, and combining the second reactant and the second solvent to form a second reactant solution, before the first reactant solution and second reactant solution are combined together to form the third reactant solution.
6. The composition of claim 1 , wherein further processing includes generally removing the second solvent by adding acetronitrile.
7. The composition of claim 1 , wherein further processing includes generally removing the first solvent by adding ethyl acetate.
8. The composition of claim 1 , wherein the second reactant includes aniline 2,5-disulfonic acid.
9. The composition of claim 1 , wherein the further processing includes substantially removing at least the first solvent and second solvent from the second solution so as to isolate the 3-phenylimino-3H-phenothiazine mediator.
10. The composition of claim 1 , wherein the 3-phenylimino-3H-phenothiazine mediator is in the form of a salt.
11. The composition of claim 1 , wherein the 3-phenylimino-3H-phenothiazine mediator has a pH in solution of from about 5 to about 8.
12. The composition of claim 11 , wherein the 3-phenylimino-3H-phenothiazine mediator has a pH in solution of from about 5.5 to about 7.
13. The composition of claim 12 , wherein the 3-phenylimino-3H-phenothiazine mediator has a pH in solution of from about 6 to about 7.
14. The composition of claim 11 , wherein the 3-phenylimino-3H-phenothiazine mediator has a pH of from about 5.5 to about 6.
15. The composition of claim 1 , further including a neutralizing agent.
16. The composition of claim 15 , wherein the neutralizing agent is sodium hydroxide.
17. The composition of claim 15 , wherein the neutralizing agent is sodium bicarbonate.
18. The composition of claim 1 , further including a buffer.
19. A composition including 3-phenylimino-3H-phenoxazine mediator formed by a process comprising the acts of:
providing a first reactant including phenoxazine;
providing a first solvent in which the phenoxazine has a desired solubility therein;
providing a second reactant to assist in forming the 3-phenylimino-3H-phenoxazine mediator;
providing a second solvent in which the second reactant has a desired solubility therein;
combining the first reactant, first solvent, second reactant and second solvent to form a third reactant solution;
adding sodium persulfate to the reactants solution to couple the first and second reactants resulting in a reaction solution including the 3-phenylimino-3H-phenoxazine mediator; and
after adding the sodium persulfate, further processing the third reactant solution including the 3-phenylimino-3H-phenoxazine mediator so as to isolate the 3-phenylimino-3H-phenoxazine mediator.
20. The composition of claim 19 , wherein the first solvent includes tetrahydrofuran (THF).
21. The composition of claim 19 , wherein the second solvent includes water.
22. The composition of claim 21 , wherein the second solvent further includes sodium hydroxide.
23. The composition of claim 19 , wherein combining the first reactant, first solvent, second reactant and second solvent including the acts of combining the first reactant and the first solvent to form a first reactant solution, and combining the second reactant and the second solvent to form a second reactant solution, before the first reactant solution and second reactant solution are combined together to form the third reactant solution.
24. The composition of claim 19 , wherein further processing includes generally removing residual second solvent by adding acetronitrile.
25. The composition of claim 19 , wherein further processing includes generally removing the first solvent by adding ethyl acetate.
26. The composition of claim 19 , wherein the further processing includes substantially removing at least the first solvent and second solvent from the second solution so as to isolate the 3-phenylimino-3H-phenoxazine mediator.
27. The composition of claim 19 , wherein the 3-phenylimino-3H-phenoxazine mediator is in the form of a salt.
28. The composition of claim 19 , wherein the 3-phenylimino-3H-phenoxazine mediator is in the form of an acid.
29. The composition of claim 19 , wherein the 3-phenylimino-3H-phenoxazine mediator has a pH in solution of from about 5 to about 8.
30. The composition of claim 29 , wherein the 3-phenylimino-3H-phenoxazine mediator has a pH in solution of from about 5.5 to about 7.
31. The composition of claim 30 , wherein the 3-phenylimino-3H-phenoxazine mediator has a pH in solution of from about 6 to about 7.
32. The composition of claim 29 , wherein the 3-phenylimino-3H-phenoxazine mediator has a pH in solution of from about 5.5 to about 6.
33. The composition of claim 19 , further including a neutralizing agent.
34. The composition of claim 33 , wherein the neutralizing agent is sodium hydroxide.
35. The composition of claim 33 , wherein the neutralizing agent is sodium bicarbonate.
36. The composition of claim 19 , further including a buffer.