Cross-reactive determinants and methods for their identification
Compositions and methods for determining immunologically cross-reactive molecules comprising a cross-reactive antigenic determinant are provided, in particular for determining proteins comprising cross-reactive antigenic determinants, in particular for determining proteins that are cross-reactive based on serological screens using sequential immunological challenges to an animal, including determining cross-reactive H. parasuis proteins. Also provided are compositions, vaccines, and kits using the molecules for diagnostics and methods for preventing or treating a disease, disorder, condition, or symptoms thereof associated with infectious agents, in particular infectious microorganisms, in particular for preventing or treating a disease, disorder, condition, or symptom thereof associated with H. parasuis infection.
1. A method for indicating presence of a cross-reactive antigenic determinant on two or more molecules, the method comprising the steps of:
Obtaining at least one antibody from an animal exposed to a first immunological challenge comprising a first antigenic determinant followed by a second immunological challenge comprising a second antigenic determinant,
wherein the first antigenic determinant is expressed by a first H. parasuis microorganism and the second antigenic determinant is expressed by a second H. parasuis microorganism, and
wherein the first and second H. parasuis microorganisms are characterized as being different serotypes;
contacting the at least one antibody with a first antigenic determinant and detecting binding; and
contacting the at least one antibody with a second antigenic determinant and detecting binding,
wherein binding of the at least one antibody to both the first and the second antigenic determinant is indicative of antibody cross-reactivity between antigenic determinants of two different H. parasuis serotypes.
2. The method of claim 1 , wherein the first H. parasuis microorganism is serotype 5, and wherein the second H. parasuis microorganism is serotype 13.
3. The method of claim 1 , wherein at least one of the first and the second antigenic determinants is present in a heme-binding protein.
4. The method of claim 1 , wherein the first and the second antigenic determinants comprise an amino acid sequence selected from the group consisting of:
(SEQ ID NO: 1)
ELANAI;
(SEQ ID NO: 2)
TVLAEKQEII;
(SEQ ID NO: 3)
APAKGSTIEAGIAYPIST
(SEQ ID NO: 4)
MKNLISI;
and
(SEQ ID NO: 5)
SPSDKTFKISAIPDYNAAEMT.
5. The method of claim 1 , wherein the animal is a porcine.