Mass spectrometric measurement of microbial resistances
View Patent ↗Microorganisms, particularly bacteria, are identified and characterized on the basis of a mass spectrometric measurement of their protein profiles with ionization by matrix-assisted laser desorption. In order to measure the microbial resistance to antibiotics, the protein profiles of microorganisms are measured after cultivation for a short time duration in nutrient media containing the antibiotics.
1. A method for determining the resistance of microbes to an antibiotic, comprising:
cultivating a sample of the microbes in a nutrient medium containing the antibiotic;
acquiring a mass spectrum of the cultivated sample;
comparing the mass spectrum to reference mass spectra of resistant and/or non-resistant microbes wherein the reference mass spectra comprise spectra of microbes different than the microbes of the sample and are acquired after the antibiotic has acted on the resistant and/or non-resistant microbes; and
determining the microbes to be resistant if the mass spectrum is similar to a reference mass spectrum of a resistant microbe or to be non-resistant if the mass spectrum is similar to a reference mass spectrum of a non-resistant microbe.
2. The method of claim 1 , wherein the sample of the microbes is cultivated a liquid nutrient medium.
3. The method of claim 2 , wherein the liquid nutrient medium is contained in a microvessel of a microtitration plate.
4. The method of claim 2 , wherein the microbes are removed from the liquid nutrient medium by filtration or centrifuging prior to acquiring the mass spectrum of the removed microbes.
5. The method of claim 4 , wherein the removed microbes are applied to a mass spectrometric support and a matrix solution is sprinkled onto the microbes on the support.
6. The method of claim 4 , wherein the removed microbes are mixed with a matrix solution and wherein the mixture is applied to a mass spectrometric sample support and dried on the mass spectrometric sample support.
7. The method of claim 1 , wherein the sample of the microbes is taken from a homogeneous colony.