Recombinant microorganisms and methods of use thereof
View Patent ↗The invention relates to methods for the production of chemical compounds, particularly but not exclusively ethanol, by microbial fermentation. Also described are genetically modified micro-organisms capable of using carbon monoxide to produce one or more products, particularly but not exclusively ethanol as a main product, and producing a reduced amount or substantially no 2,3-butanediol and/or a precursor thereof.
1. A method of producing a product comprising culturing a recombinant carboxydotrophic acetogenic microorganism in the presence of a gaseous substrate to produce at least one product, wherein the microorganism comprises at least one genetic modification that disrupts the expression and/or activity of an enzyme in the 2,3-butanediol biosynthesis pathway selected from the group consisting of (i) an enzyme capable of converting pyruvate to acetolactate, (ii) an enzyme capable of converting acetolactate to acetoin, and (iii) an enzyme capable of converting acetoin to 2,3-butanediol.
2. The method of claim 1 , wherein the product is selected from the group consisting of ethanol, formate, lactate, pyruvate, succinate, valine, leucine, isoleucine, acetolactate, malate, fumarate, 2-oxogluterate, and citrate.
3. The method of claim 2 , wherein the product is ethanol.
4. The method of claim 1 , wherein the microorganism comprises at least one genetic modification that disrupts the expression and/or activity of at least two enzymes in the 2,3-butanediol biosynthesis pathway selected from the group consisting of (i) an enzyme capable of converting pyruvate to acetolactate, (ii) an enzyme capable of converting acetolactate to acetoin, and (iii) an enzyme capable of converting acetoin to 2,3-butanediol.
5. The method of claim 1 , wherein the enzyme capable of converting pyruvate to acetolactate is acetolactate synthase.
6. The method of claim 5 , wherein the acetolactate synthase is AlsS.
7. The method of claim 1 , wherein the enzyme capable of converting acetolactate to acetoin is acetolactate decarboxylase.
8. The method of claim 7 , wherein the acetolactate decarboxylase is BudA.
9. The method of claim 1 , wherein the enzyme capable of converting acetoin to 2,3-butanediol is 2,3-butanediol dehydrogenase, acetoin reductase, or primary:secondary alcohol dehydrogenase.
10. The method of claim 9 , wherein the 2,3-butanediol dehydrogenase is 2,3-Bdh.
11. The method of claim 1 , wherein the microorganism produces an increased amount of the product compared to a parental microorganism and/or a reduced amount of 2,3-butanediol or a precursor thereof compared to a parental microorganism.
12. The method of claim 11 , wherein the parental microorganism is Clostridium autoethanogenum, Clostridium ljungdahlii, Clostridium ragsdalei , or Clostridium coskatii.
13. The method of claim 11 , wherein the parental microorganism is Clostridium Autoethanogenum DSM23693.
14. The method of claim 1 , wherein the gaseous substrate is an industrial waste gas or an industrial off gas.
15. The method of claim 1 , wherein the gaseous substrate is syngas.
16. The method of claim 1 , wherein the gaseous substrate comprises CO.
17. The method of claim 16 , wherein the gaseous substrate comprises at least 20% CO by volume.