Media for culturing, preserving, and administering regenerative cells
View Patent ↗A culture media, media supplement, or soluble matrix for cryopreservation or enhanced regenerative cell growth in culture and maintenance of multi-lineage differentiation potentiation. The inventive culture media, media supplement, or soluble matrix comprises a GAG composition comprising a sulfated GAG, such as chondroitin sulfate. A soluble matrix, a cell administration package or kit comprising the soluble matrix and a device for cell administration, and a method of use thereof, for administration of regenerative cells for treating a joint disease or other weakened or damaged tissue comprising the specified GAG compositions are further provided.
1. A method of culturing cells having multi-lineage differentiation potential, comprising culturing the cells for at least 24 hours in a cell culture media comprising a glycosaminoglycan (GAG) composition, wherein said GAG composition comprises hyaluronic acid, chondroitin sulfate and N-acetyl D-glucosamine in a relative weight ratio of 1:20:20, wherein said GAG composition is at a concentration of 5-10% (v/v), and wherein the cultured cells have an enhanced in vitro expansion rate and maintain multi-lineage differentiation potential compared to cells having a multi-lineage differentiation potential not cultured in the media.
2. The method of claim 1 , wherein said composition is at a concentration of 5-7% (v/v).
3. The method of claim 2 , wherein said composition is at a concentration of 5% (v/v).
4. The method of claim 1 , wherein said GAG composition comprises hyaluronic acid, CS4 and CS6 chondroitin sulfate and N-acetyl D-glucosamine at a concentration 5-10% (v/v).
5. The method of claim 1 , wherein said cells are treated with a media supplement comprising the GAG composition and then culturing the cells.
6. The method of claim 1 , wherein said cells are grown on a surface coated with a soluble matrix comprising the GAG composition.
7. The method of claim 1 , wherein said cell is an adipose-derived mesenchymal stem cell (Ad-MSC).
8. The method of claim 1 , wherein the cells are cultured for at least 3 days.
9. The method of claim 1 , wherein the cells are cultured for at least 6 days.
10. The method of claim 1 , wherein the GAG composition comprises about 5 mg/ml hyaluronic acid sodium salt, about 100 mg/ml sodium chondroitin sulfate, and about 100 mg/ml N-acetyl D-glucosamine.