EICOSAPENTAENOIC ACID-PRODUCING MICROORGANISMS, FATTY ACID COMPOSITIONS, AND METHODS OF MAKING AND USES THEREOF
The present invention is directed to isolated microorganisms as well as strains and mutants thereof, biomasses, microbial oils, compositions, and cultures; methods of producing the microbial oils, biomasses, and mutants; and methods of using the isolated microorganisms, biomasses, and microbial oils.
1 .- 46 . (canceled)
47 . An animal feed comprising a microbial oil comprising at least about 20% by weight eicosapentaenoic acid and less than about 5% by weight each of arachidonic acid, docosapentaenoic acid n-6, oleic acid, linoleic acid, linolenic acid, eicosenoic acid, erucic acid, and stearidonic acid.
48 . The animal feed of claim 47 , further comprising at least about 25% by weight docosahexaenoic acid.
49 . An animal feed comprising a biomass, wherein at least about 20% by weight of a dry cell weight of the biomass are fatty acids, wherein more than about 10% by weight of fatty acids is eicosapentaenoic acid, and wherein the fatty acids comprise less than about 5% by weight each of arachidonic acid and docosapentaenoic acid n-6.
50 . The animal feed of claim 49 , wherein at least about 25% by weight of the fatty acids is docosahexaenoic acid.
51 . An animal feed comprising a biomass, wherein at least about 12% by weight of triacylglycerol is eicosapentaenoic acid.
52 . An animal feed comprising a microbial oil comprising a triacylglycerol fraction which is at least 10% by weight of said microbial oil, wherein at least 12% by weight of the fatty acids in the triacylglycerol fraction is eicosapentaenoic acid, wherein at least 20% by weight of the fatty acids in the triacylglycerol fraction is docosahexaenoic acid, and wherein less than 5% by weight of the fatty acids in the triacylglycerol fraction is arachidonic acid.
53 . The animal feed of claim 52 , wherein the triacylglycerol fraction is at least 40% by weight.
54 . The animal feed of claim 52 , wherein at least 15% by weight of the fatty acids in the triacylglycerol fraction is eicosapentaenoic acid.
55 . The animal feed of claim 52 , wherein at least 25% by weight of the fatty acids in the triacylglycerol fraction is docosahexaenoic acid.
56 . The animal feed of claim 52 , wherein 2% or less by weight of the fatty acids in the triacylglycerol fraction is arachidonic acid.
57 . The animal feed of any one of claims 49 to 56 , wherein the fatty acids comprise less than about 5% by weight each of oleic acid, linoleic acid, linolenic acid, eicosenoic acid, and erucic acid.
58 . An animal feed comprising a microorganism of the species deposited under ATCC Accession No. PTA-10208, wherein the total fatty acids produced by the microorganism comprises more than about 10% by weight eicosapentaenoic acid.
59 . An animal feed comprising a microorganism having the characteristics of the species deposited under ATCC Accession No. PTA-10208, wherein the total fatty acids produced by the microorganism comprises more than about 10% by weight eicosapentaenoic acid.
60 . An animal feed comprising a microorganism that produces a triacylglycerol fraction, wherein eicosapentaenoic acid content of the triacylglycerol fraction is at least about 12% by weight.
61 . The animal feed of any one of claims 59 - 60 , wherein the microorganism is a mutant strain.
62 . An animal feed comprising one or more microorganism selected from the group consisting of: PTA-10208, PTA-10209, PTA-10210, and PTA-10211.
63 . The animal feed of any one of claims 58 to 62 , wherein the microorganism is in the form of biomass.
64 . The animal feed of claims 47 to 63 , wherein the animal feed is an aquaculture feed.
65 . The animal feed of claims 47 to 63 , wherein the animal feed is a domestic animal feed, a zoological animal feed, a work animal feed, a livestock feed, or a combination thereof.
66 . Use of the animal feed of any one of claims 47 to 65 for providing nutrition to animals.