Method for analysis using X-ray fluorescence
View Patent ↗The present invention is a method to quantify biomarkers. The method uses an X-ray fluorescence spectrometer to perform an X-ray fluorescence analysis on the sample to obtain spectral features derived from the biomarker; and quantifying the X-ray fluorescence signal of the biomarker.
1. A method to quantify biomarkers, comprising providing a sample comprising a biomarker, the biomarker comprising one or more chemicals that are related to a physiological condition; preparing the sample for X-ray analysis by exposing the sample to a solution; using an X-ray fluorescence spectrometer to perform an X-ray fluorescence analysis on the sample to obtain spectral features derived from the biomarker; and quantifying the X-ray fluorescence signal of the biomarker; wherein the X-ray fluorescence spectrometer irradiates the sample with polychromatic X-rays.
2. The method of claim 1 , wherein the solution is selected from the group of solution comprising a pH buffer, a redox buffer, a preservative, a fixative, and a sterilizing solution.
3. The method of claim 1 , wherein the solution is free of at least one chemical element having an atomic number greater than eight, where the element is present in the sample.
4. The method of claim 1 , wherein the solution is free of at least one of the chemicals or functional groups selected from the group consisting of dimethylsulfoxide, thiols, sulfate anion, sulfonate anions, chloride anion, bromide anion, fluoride anion, iodide anion, perchlorate anion, phosphate anion, and phosphonate anions.
5. The method of claim 1 , wherein the solution comprises one or more chemicals selected from the group consisting of amine, imine, nitrate anion, nitrite anion, ammonium cation, acetate anion, carboxylate anion, conjugate bases of carboxylic acids, carbonate anion, formalin, formaldehyde, ethanol, 2-propanol, and iminium cation.
6. A method to diagnose abnormal conditions, comprising providing one or more clinical samples, the one or more clinical samples comprising a biomarker; preparing the sample for X-ray analysis by exposing the sample to a solution; using an X-ray fluorescence spectrometer to perform an X-ray fluorescence analysis on at least one of the one or more clinical samples to obtain spectral features derived from at least one biomarker; and quantifying the X-ray fluorescence signal of the biomarker, wherein the X-ray fluorescence spectrometer irradiates the sample with polychromatic X-rays.
7. The method of claim 6 , wherein the solution is selected from the group of solutions comprising a pH buffer, a redox buffer, a preservative, a fixative, and a sterilizing solution.
8. The method of claim 6 , wherein the solution is free of at least one chemical element having an atomic number greater than eight, where the element is present in the sample.
9. The method of claim 6 , wherein the solution is free of at least one of the chemicals or functional groups selected from the group consisting of dimethylsulfoxide, thiols, sulfate anion, sulfonate anions, chloride anion, bromide anion, fluoride anion, iodide anion, perchlorate anion, phosphate anion, and phosphonate anions.
10. The method of claim 6 , wherein the solution comprises one or more chemicals selected from the group consisting of amine, imine, nitrate anion, nitrite anion, ammonium cation, acetate anion, carboxylate anion, conjugate bases of carboxylic acids, carbonate anion, formalin, formaldehyde, ethanol, 2-propanol, and iminium cation.
11. The method of claim 6 , further comprising the step of obtaining data concerning the at least one of the one or more clinical samples using at least one other test.
12. The method of claim 11 , further comprising the step of comparing the spectral features derived from the biomarker to data obtained from the at least one other test.
13. The method of claim 6 further comprising the step of performing a diagnosis.
14. A method to identify one or more abnormal portions of a sample, comprising providing a clinical sample disposed on or in a sample holder, the clinical sample comprising normal and abnormal portions; preparing the clinical sample for X-ray fluorescence analysis by exposing the clinical sample to a solution, providing an X-ray fluorescence spectrometer, the X-ray fluorescence spectrometer comprising a X-ray excitation source; using the X-ray fluorescence spectrometer to perform an X-ray fluorescence analysis on the clinical sample to obtain spectral features derived from at least one biomarker; and using spectral features derived from the biomarker to determine whether at least one portion of the sample is normal or abnormal, wherein the X-ray fluorescence is performed using excitation of the sample with polychromatic X-rays.
15. The method of claim 14 , wherein the solution is free of at least one chemical element having an atomic number greater than eight, where the element is present in the sample.
16. The method of claim 14 , wherein the solution is free of at least one of the chemicals or functional groups selected from the group consisting of dimethylsulfoxide, thiols, sulfate anion, sulfonate anions, chloride anion, bromide anion, fluoride anion, iodide anion, perchlorate anion, phosphate anion, and phosphonate anions.
17. The method of claim 14 , wherein the solution comprises one or more chemicals selected from the group consisting of amine, imine, nitrate anion, nitrite anion, ammonium cation, acetate anion, carboxylate anion, conjugate bases of carboxylic acids, carbonate anion, formalin, formaldehyde, ethanol, 2-propanol, and iminium cation.
18. The method of claim 14 , wherein the solution is selected from the group of solutions comprising a pH buffer, a redox buffer, a preservative, a fixative, and a sterilizing solution.
19. The method of claim 14 , wherein the X-ray fluorescence analysis comprises multiple X-ray fluorescence analyses obtained wherein the sample is disposed at least two different angles relative to the excitation source.
20. The method of claim 14 , further comprising the step of constructing a tomogram.