Method and composition for detecting oxidizing salts
View Patent ↗The present invention provides methods for determining the presence of oxidizing salts. According to the current invention, analyte is collected on a swipe and subsequently heated to a temperature sufficient to release a detectible vapor phase component of the oxidizing salt. The vapor phase component passes reacts with a pH sensitive molecule. Reaction of the vapor phase product with the pH sensitive molecule produces a detectible change in response intensity.
1. A detector assembly configured to detect trace amounts of evaporative dissociation compounds derived from an oxidizing salt, the detector assembly comprising:
a sensor assembly including a solid substrate carrying a pH sensitive molecule, an excitation source positioned to provide stimulating radiation to the pH sensitive molecule, a light sensor to monitor a response of the pH sensitive molecule to stimulation, and a heater suitable for adjusting the temperature of said sensor assembly;
a sampling tip assembly in fluid communication with said sensor assembly;
a heater associated with said sampling tip assembly, said heater suitable for maintaining the temperature of the sampling tip assembly at a temperature between about 90° and 350°;
a gas movement pump in fluid communication with said sampling tip and said sensor assembly, said gas movement pump configured to move vapors from said sampling tip assembly to and along said solid substrate of said sensor assembly.
2. The detector assembly of claim 1 , wherein the pH sensitive molecule is selected from the group consisting of:
SNARF®-5F, 5-(and-6)-carboxylic acid as represented by the following structure where the carboxylic acid group may be positioned at either the indicated 5 or 6 position,
SNARF®-4F, 5-(and-6)-carboxylic acid as represented by the following structure where the carboxylic acid group may be positioned at either the indicated 5 or 6 position,
5-(and-6)-carboxy SNARE®-1, as represented by the following structure where the carboxylic acid group may be positioned at either the indicated 5 or 6 position,
LysoSensor™ Green DND-153 as represented by the following structure,
2′,7′-difluorofluorescein as represented by the following structure,
2-[5-methoxy-2-(4-phenyl-quinoline-2yl)-phenyl]-ethanol as represented by the following structure
2-{2-[-(tert-butyl-dimethyl-silanyloxy)-ethyl]-4-methoxy-phenyl}-4-phenyl-quinoline as represented by the following structure
3. The detector assembly of claim 1 , wherein said pH sensitive molecule produces a detectible response when stimulated by an excitation source emitting light radiation at wavelengths between about 325 nm and about 700 nm.
4. The detector assembly of claim 1 , wherein said pH sensitive molecule produces a detectible response when reacted with an acid component of an oxidizing salt while undergoing excitation by an excitation source emitting light radiation at wavelengths between about 325 nm and about 700 nm.
5. The excitation source in any of claims 3 , wherein said excitation source emitting light radiation at wavelengths between about 365 nm and about 410 nm.
6. The excitation source in any of claims 4 , wherein said excitation source emitting light radiation at wavelengths between about 365 nm and about 410 nm.