High-throughput sample processing systems and methods of use
View Patent ↗Disclosed herein are high-throughput sample processing systems and waste management systems, and methods of using the same.
1. A high throughput sample processing system comprising:
a sample dispensing device for drawing a plurality of samples from a plurality of sample containers and for dispensing each sample into a well of a sample processing plate comprising a plurality of wells, wherein each sample is dispensed into a different well wherein the sample in each well comprises a meniscus;
a contactless fluid dispensing device for dispensing fluids into the plurality of wells of the sample processing plate, said contactless fluid dispensing device being configured to dispense fluid into a plurality of wells of said sample processing plate without contacting liquids already in the wells;
a plurality of contactless liquid level sensors for detecting the liquid level in each of the plurality of wells of the sample processing plate, said contactless liquid level sensors being configured to determine the liquid level without contacting liquid already in the wells;
a plurality of aspirators for removing fluids from the plurality of wells of the sample processing plate, said aspirators configured to maintain an approximately equal distance from the tip of the fluid aspirating nozzles and the sample meniscus, and further wherein the aspirators are connected to a waste management system;
a plurality of contactless treatment stations for treating a plurality of sample processing plates simultaneously, said contactless treatment stations are selected from an orbital shaker, a thermo shaker, a magnetic station, a water bath, a heating block, a chilling block, an ambient incubator and combinations thereof;
a waste management system for managing fluids removed from the plurality of wells; and
a control system for controlling the processing of a plurality of plates within the high throughput sample processing system simultaneously, wherein the control system is operatively connected to said sensors, dispensing devices, aspirators, and treatment stations, and is programmed to dynamically control the dispensing devices and aspirators such that the dispensing device and the aspirators do not touch the sample meniscus of each well, and the suction forces provided by the aspirators are strong enough to cause liquid to be pulled into the aspirators.
2. The high throughput sample processing system of claim 1 , wherein the control system is further configured to dynamically control the processing of a plate out of the plurality of sample processing plates depending upon the location or status of other plates in the system.
3. The high throughput sample processing system of claim 1 , further comprising a plate loading device for automatically loading additional sample processing plates into the sample dispensing device.
4. The high throughput sample processing system of claim 1 , wherein the sample dispensing device comprises a plurality of syringe based pipettes.
5. The high throughput sample processing system of claim 4 , wherein the pipettes comprise reusable pipette tips.
6. The high throughput sample processing system of claim 5 , wherein the sample dispensing device comprises a washing station for automatically washing the reusable pipette tips.
7. The high throughput sample processing system of claim 6 , wherein the washing station is configured to contain a bleach solution.
8. The high throughput sample processing system of claim 1 , wherein the liquid level sensors comprise one or more acoustic sensors.
9. The high throughput sample processing system of claim 1 , wherein the waste management system comprises a waste container, and further wherein the waste management system is configured to deposit the fluids removed from the plurality of wells into said waste container using gravity or suction forces, and further wherein the waste container can comprise a sterilization solution.
10. The high throughput sample processing system of claim 9 , wherein the waste container is configured to operate under a vacuum.
11. The high throughput sample processing system of claim 9 , wherein the waste management system is configured to mix the fluids removed from the plurality of wells with bleach in said waste container and incubates the mixture.
12. The high throughput sample processing system of claim 1 , wherein the waste management system comprises one or more scales for determining an amount of fluids removed from the plurality of wells.
13. The high throughput sample processing system of claim 1 , comprising a barcode scanner configured for identifying samples using barcodes on the sample containers.
14. A waste management system for processing waste produced by a high-throughput sample processing system according to claim 1 , wherein the waste management system comprises:
a gravity-based liquid waste input;
a vacuum-based liquid waste input;
a sterilizing fluid container operatively connected to at least one liquid waste container;
two or more liquid waste containers, configured to alternatively accept liquid waste from said inputs, treat the liquid waste with a sterilizing fluid from said sterilizing fluid container, and incubate the sterilizing fluid in the liquid waste for a predetermined period of time before disposing of the treated liquid waste; and
one or more scales for determining the amount of liquid waste collected by the one or more liquid waste containers.