Method for forming an optical test sensor
View Patent ↗Systems and methods for electrochemically oxidizing components of a test-sensor reagent prior to deposition on a test sensor comprise at least a first electrode and a second electrode for contacting the test-sensor reagent. The first electrode and the second electrode may have hollow interior portions for contacting the test-sensor reagent to produce a modified test-sensor reagent having a reduced background current.
1. A method for forming an optical test sensor to be used with an optical analysis system, the method comprising the acts of:
providing a base and a second layer, the base and the second layer assisting in forming a channel for receiving a fluid sample;
providing a reagent material with an oxidizable species therein;
applying voltage to the reagent material via an electrochemical system to oxidize the oxidizable species therein and form a modified reagent material having a reduced background current as compared to a reagent material where voltage has not been applied via an electrochemical system; and
placing the modified reagent material to assist in forming the optical test sensor, the modified reagent material being placed in the channel of the optical test sensor so as to contact the fluid sample,
wherein the reagent material includes a mediator and an enzyme.
2. The method of claim 1 , wherein the mediator is 3-phenylimino-3H-phenothiazine or 3-phenylimino-3H-phenoxazine.
3. The method of claim 1 , wherein the enzyme includes glucose oxidase.
4. The method of claim 1 , wherein enzyme includes glucose dehydrogenase.
5. The method of claim 1 , wherein the reagent further includes a binder.
6. The method of claim 5 , wherein the binder is a cellulose polymer.
7. The method of claim 1 , wherein the reagent further includes a surfactant.
8. The method of claim 1 , wherein applying potential to the reagent material to oxidize the oxidizable species therein includes flowing the reagent material through a system comprising a first electrode and a second electrode and applying the potential to at least one of the first electrode or the second electrode to oxidize any oxidizable species in the reagent material.
9. The method of claim 1 , wherein the second layer is a spacer, a lid or a combination thereof.
10. A method for forming an optical test sensor to be used with an optical analysis system, the method comprising the acts of:
providing a base and a second layer, the base and the second layer assisting in forming a channel for receiving a fluid sample;
providing a reagent material with an oxidizable species therein;
applying voltage to the reagent material via an electrochemical system to oxidize the oxidizable species therein and form a modified reagent material having a reduced background current as compared to a reagent material where voltage has not been applied via an electrochemical system; and
placing the modified reagent material to assist in forming the optical test sensor, the modified reagent material being placed in the channel of the optical test sensor so as to contact the fluid sample,
wherein the reagent material includes a mediator, glucose dehydrogenase and cellulose polymer.
11. The method of claim 10 , wherein the mediator is 3-phenylimino-3H-phenothiazine or 3-phenylimino-3H-phenoxazine.
12. The method of claim 10 , wherein the reagent further includes a surfactant.
13. The method of claim 10 , wherein applying potential to the reagent material to oxidize the oxidizable species therein includes flowing the reagent material through a system comprising a first electrode and a second electrode and applying the potential to at least one of the first electrode or the second electrode to oxidize any oxidizable species in the reagent material.