Fluid sample preparation systems and methods
Sample application systems can include an extraction mechanism to remove a sample from sample containers, a sample vessel disposed on a deployment mechanism, where the deployment mechanism is arranged to move the sample vessel to receive a sample, an extraction mechanism washing station to wash the extraction mechanism, a sample applicator to remove a portion of the sample in the sample vessel and apply it onto a sample carrier, where the deployment mechanism can move the sample vessel to a sample application position, a sample vessel washing station to wash the sample vessel, where the deployment mechanism can move the sample vessel to the sample vessel washing station, a sample applicator washing station to wash the sample applicator after the sample has been dispensed onto the sample carrier, and a fluid control system to control flow of a fluid provided to the extraction mechanism and the sample applicator.
1. A method of handling a sample, the method comprising:
receiving a sample container containing a volume of a sample;
removing a sample from the sample container using an extraction device;
dispensing the sample into a sample vessel with the extraction device;
washing the extraction device by dispensing a fluid through the extraction device;
moving the sample vessel containing the sample to a sample application position;
removing a portion of the sample from the sample vessel using a sample applicator;
dispensing the sample portion from the sample applicator to directly contact a surface of a sample carrier, wherein the sample portion is dispensed onto the surface in at least one of a serpentine pattern, a raster pattern, a spiral pattern, a pattern of concentric circles, and a pattern of multiple lines;
rinsing the sample applicator by dispensing fluid through the sample applicator; and
washing the sample vessel to remove any residual sample.
2. The method of claim 1 , wherein the extraction device is operated by a fluid system, and wherein fluid of the fluid system is separated from the sample by an air pocket within the extraction device.
3. The method of claim 1 , further comprising modifying the sample dispensed into the sample vessel.
4. The method of claim 3 , wherein modifying the sample comprises adding a diluent fluid to the sample in the sample vessel.
5. The method of claim 1 , wherein removing a sample from the sample container comprises inserting a needle through a cap attached to a test tube.
6. The method of claim 1 , wherein the extraction device and the sample applicator retain the sample when the fluid control system generates a vacuum in the extraction device and the sample applicator.
7. The method of claim 1 , wherein rinsing the extraction device and rinsing the sample applicator comprises inserting a portion of the extraction device and a portion of the sample applicator into respective receptacles having curved bottoms, such that fluid dispensed from the extraction device and the sample applicator is directed along an outer surface of the extraction device and the sample applicator.
8. The method of claim 1 , wherein dispensing the sample portion from the sample applicator comprises dispensing the sample portion onto a glass slide.
9. The method of claim 1 , wherein the sample comprises a body fluid.
10. The method of claim 9 , wherein the body fluid is blood.
11. The method of claim 1 , further comprising rotating the sample container from a first position to a second position angularly displaced from the first position to agitate the sample.
12. The method of claim 11 , further comprising rotating the sample container from the first position to the second position multiple times to agitate the sample.
13. The method of claim 1 , further comprising inverting the sample container so that the extraction device removes the sample from the sample container when the sample container is inverted.
14. The method of claim 13 , further comprising inverting the extraction device prior to removing the sample from the sample container.