Test cartridge with integrated transfer module
A system that includes a cartridge housing and a hollow transfer module, according to an embodiment is described herein. The cartridge housing further includes at least one sample inlet, a plurality of storage chambers, a plurality of reaction chambers, and a fluidic network. The fluidic network is designed to connect the at least one sample inlet, a portion of the plurality of storage chambers and the portion of the plurality of reaction chambers to a first plurality of ports located on an inner surface of the cartridge housing. The hollow transfer module includes a second plurality of ports along an outer surface of the transfer module that lead to a central chamber within the transfer module. The transfer module is designed to move laterally within the cartridge housing. The lateral movement of the transfer module aligns at least a portion of the first plurality of ports with at least a portion of the second plurality of ports.
1. A method comprising:
laterally translating a transfer module to align a first port of the transfer module having a central chamber to a port of a first chamber;
drawing a sample into the central chamber from the first chamber via a first pressure differential applied to the sample;
laterally translating the transfer module to align a second port of the transfer module to a port of a second chamber; and
drawing the sample into the second chamber from the central chamber via a second pressure differential applied to the sample.
2. The method of claim 1 , further comprising mixing the sample introduced into the first chamber with a buffer disposed within the first chamber.
3. The method of claim 1 , further comprising mixing the sample drawn into the central chamber from the first chamber with a liquid already present in the central chamber.
4. The method of claim 1 , further comprising: introducing the sample to the first chamber via a swab carrying the sample.
5. The method of claim 1 , further comprising:
processing the sample in the second chamber;
drawing the sample into the central chamber from the second chamber via a third pressure differential applied to the sample;
laterally translating the transfer module to align the second port of the transfer module to a port of a third chamber;
drawing the sample into the third chamber from the central chamber via a fourth pressure differential applied to the sample; and
measuring one or more qualities of the sample while in the third chamber.
6. The method of claim 5 , wherein measuring comprises optically measuring a fluorescence signal.
7. The method of claim 5 , wherein measuring comprises optically measuring an absorbance.
8. The method of claim 5 , further comprising heating the sample after either drawing the sample into the second chamber or drawing the sample into the third chamber.
9. The method of claim 1 , further comprising repeating laterally translating the transfer module to align the first port of the transfer module with various ports of one or more chambers.
10. The method of claim 9 , further comprising drawing one or more liquids into the central chamber from the one or more chambers via one or more pressure differentials.
11. The method of claim 1 , further comprising repeating laterally translating the transfer module to align the second port of the transfer module with various ports of one or more chambers.
12. The method of claim 11 , further comprising drawing one or more liquids into the central chamber from the one or more chambers via one or more pressure differentials.
13. The method of claim 11 , further comprising drawing one or more liquids into the one or more chambers from the central chamber via one or more pressure differentials.
14. The method of claim 1 , further comprising:
processing the sample in the second chamber;
drawing the sample into the central chamber from the second chamber via a third pressure differential applied to the sample;
laterally translating the transfer module to align the second port of the transfer module to a port of a third chamber;
drawing the sample into the third chamber from the central chamber via a fourth pressure differential applied to the sample;
processing the sample in the third chamber;
laterally translating the transfer module to align the second port of the transfer module to a port of a fourth chamber;
drawing the sample into the fourth chamber from the central chamber via a fifth pressure differential applied to the sample; and
measuring one or more qualities of the sample while in the fourth chamber.
15. The method of claim 1 , wherein drawing the sample into the second chamber comprises flowing the sample through a filter.
16. The method of claim 1 , wherein drawing the sample into the second chamber comprises flowing the sample through a fluid splitter into one or more sub-chambers.
17. The method of claim 1 , further comprising mixing the sample within the central chamber with a magnetic stir bar.