Plurality of reaction chambers in a test cartridge
A fluidic testing system is presented that includes a plurality of test chambers, a plurality of inlet channels, and a fluidic network that connects the inlet channels to one or more other chambers. The plurality of test chambers are each characterized by a length and a hydraulic diameter. The length of each test chamber is aligned substantially parallel to a gravity vector. Each of the test chambers has only one opening disposed along the length of the corresponding test chamber. Additionally, each of the test chambers is coupled via its respective opening to only one of the plurality of inlet channels.
1. A method comprising:
flowing liquid through each of a plurality of channels up to a liquid sensing area disposed in each channel, thus setting a pre-determined amount of liquid within each of the plurality of channels, each of the plurality of channels coupled to an opening of its own corresponding chamber of a plurality of chambers, wherein each of the chambers has a wall that defines a longest side of the chamber, and wherein the opening of each of the chambers is the only opening through the wall of each of the chambers;
simultaneously flowing only the pre-determined amount of liquid within each of the plurality of channels into each of the chambers.
2. The method of claim 1 , wherein one or more of the plurality of chambers includes one or more reagents, and the method further comprises re-suspending the one or more reagents in the pre-determined amount of liquid.
3. The method of claim 1 , further comprising re-suspending one or more reagents within premixing chambers coupled to each of the plurality of channels.
4. The method of claim 1 , further comprising heating at least one of the plurality of chambers.
5. The method of claim 4 , wherein the heating comprises heating with a Peltier device.
6. The method of claim 4 , wherein the heating comprises heating with resistive heating elements.
7. The method of claim 4 , wherein the heating comprises heating with forced air.
8. The method of claim 1 , further comprising detecting one or more optical properties of contents in the liquid within at least one of the chambers.
9. The method of claim 1 , wherein the flowing is performed via a single pump source.