Methods and compositions for highly sensitive detection of molecules
Disclosed are methods, kits, and compositions for the highly sensitive detection of molecules. The methods, kits, and compositions are useful in determining concentrations of molecules in samples to levels of 1 femtomolar, 1 attomolar, or lower. The methods, kits, and compositions also allow the determination of concentration over a wide range, e.g., 7-log range, without need for sample dilution.
1. An analyzer comprising:
a) an electromagnetic radiation source for stimulating a fluorescent moiety;
b) a capillary flow cell for passing said label;
c) a source of motive force for moving said label in said capillary flow cell;
d) an interrogation space defined within said capillary flow cell for receiving electromagnetic radiation emitted from said electromagnetic source; and
e) an electromagnetic radiation detector operably connected to said interrogation space for measuring an electromagnetic characteristic of a stimulated fluorescent moiety;
a processor operatively connected to the detector, wherein the processor is configured to execute instructions stored on a non-transitory computer-readable medium, and wherein the instructions, when executed by the processor, cause the processor to:
determine a threshold photon value corresponding to a background signal in the interrogation space,
determine the presence of a fluorescent moiety in the interrogation space in each of a plurality of bins by identifying bins having a photon value greater than the threshold value, and
compare the number of bins having a photon value greater than the threshold value to a standard curve.
2. The analyzer system of claim 1 , wherein said electromagnetic radiation source is a laser, and wherein said laser has a power output of at least about 3, 5, 10, or 20 mW.
3. The analyzer system kit of claim 1 , wherein the analyzer comprises not more than one interrogation space.
4. The analyzer system of claim 1 wherein said electromagnetic radiation source is a continuous wave electromagnetic radiation source.
5. The analyzer system of claim 4 , wherein said continuous wave electromagnetic radiation source is a light-emitting diode or a continuous wave laser.
6. The analyzer system of claim 1 , wherein said motive force is pressure.
7. The analyzer system of claim 1 , wherein said detector is an avalanche photodiode detector.
8. The system of claim 1 , further comprising a confocal optical arrangement for deflecting a laser beam onto said interrogation space and for imaging a stimulated fluorescent moeity, wherein said confocal optical arrangement comprises an objective lens having a numerical aperture of at least about 0.8.
9. The analyzer system of claim 1 further comprising a sampling system capable of automatically sampling a plurality of samples and providing a fluid communication between a sample container and said interrogation space.
10. The analyzer system of claim 1 further comprising a sample recovery system in fluid communication with said interrogation space, wherein said recovery system is capable of recovering substantially all of said sample.