Plurality of reaction chambers in a test cartridge
A fluidic testing system is presented that includes a plurality of test chambers, a plurality of inlet channels, and a fluidic network that connects the inlet channels to one or more other chambers. The plurality of test chambers are each characterized by a length and a hydraulic diameter. The length of each test chamber is aligned substantially parallel to a gravity vector. Each of the test chambers has only one opening disposed along the length of the corresponding test chamber. Additionally, each of the test chambers is coupled via its respective opening to only one of the plurality of inlet channels.
1. A method comprising:
flowing liquid through a plurality of inlet channels, each inlet channel coupled to a corresponding test chamber of a plurality of test chambers via an opening through a wall of the corresponding test chamber, wherein the wall defines a longest side of the corresponding test chamber, and wherein the opening is the only opening into the corresponding test chamber;
filling each of the plurality of test chambers with the liquid up to a threshold amount;
drawing the liquid away from each of the plurality of test chambers through the opening of each corresponding test chamber, wherein the drawing leaves behind a predetermined amount of the liquid below a height at which the opening is disposed along the wall of each corresponding test chamber.
2. The method of claim 1 , further comprising re-suspending one or more reagents disposed in one or more of the plurality of test chambers in the predetermined amount of the liquid left behind within the one or more test chambers.
3. The method of claim 2 , wherein the drawing draws liquid that does not contain the one or more reagents.
4. The method of claim 1 , wherein filling up to the threshold amount comprises filling each of the test chambers up to a height equal to or greater than a height at which the opening is disposed along the wall of each corresponding test chamber.
5. The method of claim 1 , further comprising heating the liquid within at least one of the plurality of test chambers.
6. The method of claim 5 , wherein the heating comprises heating with a Peltier device.
7. The method of claim 5 , wherein the heating comprises heating with resistive heating elements.
8. The method of claim 5 , wherein the heating comprises heating with forced air.
9. The method of claim 1 , further comprising detecting one or more optical properties of contents in the liquid within at least one of the plurality of test chambers.
10. The method of claim 1 , wherein the flowing is performed via a single pump source.