Genetically engineered bacterium with altered carbon monoxide dehydrogenase (CODH) activity
View Patent ↗The invention provides genetically engineered microorganisms with altered carbon monoxide dehydrogenase (CODH) activity and methods related thereto. In particular, the invention provides a genetically engineered carboxydotrophic acetogenic bacterium having decreased or eliminated activity of CODH1 and/or CODH2. In certain embodiments, the bacterium may also have increased activity of CODH/ACS. The invention further provides a method for producing a product by culturing the bacterium in the presence of a gaseous substrate comprising one or more of carbon monoxide, carbon dioxide, and hydrogen.
1. A genetically engineered carboxydotrophic acetogenic bacterium having decreased or eliminated activity of CODH1 and/or CODH2 compared to a parental bacterium.
2. The bacterium of claim 1 , wherein the bacterium comprises at least one disruptive mutation in a CODH1 gene and/or CODH2 gene.
3. The bacterium of claim 2 , wherein the disruptive mutation decreases or eliminates expression of the CODH1 gene and/or the CODH2 gene compared to a parental bacterium.
4. The bacterium of claim 2 , wherein the disruptive mutation is a knockout mutation.
5. The bacterium of claim 1 , wherein the bacterium additionally has increased activity of CODH/ACS compared to the parental bacterium.
6. The bacterium of claim 5 , wherein the bacterium overexpresses a CODH/ACS gene compared to the parental bacterium.
7. The bacterium of claim 1 , wherein the bacterium produces one or more of ethanol and 2,3-butanediol.
8. The bacterium of claim 1 , wherein the bacterium produces a higher amount of ethanol, produces a lower amount of acetate, has a shorter lag phase, and/or has a higher growth rate compared to the parental bacterium.
9. The bacterium of claim 1 , wherein the bacterium consumes a gaseous substrate comprising one or more of CO, CO 2 , and H 2 .
10. The bacterium of claim 1 , wherein the parental bacterium is Clostridium autoethanogenum, Clostridium ljungdahlii , or Clostridium ragsdalei.
11. A method for producing a product, comprising culturing the bacterium of claim 1 in the presence of a gaseous substrate comprising one or more of CO, CO 2 , and H 2 , whereby the bacterium produces a product.
12. The method of claim 11 , wherein the bacterium comprises at least one disruptive mutation in a CODH1 gene and/or CODH2 gene.
13. The method of claim 12 , wherein the disruptive mutation decreased or eliminates expression of the CODH1 gene and/or the CODH2 gene compared to a parental bacterium.
14. The method of claim 12 , wherein the disruptive mutation is a knockout mutation.
15. The method of claim 11 , wherein the bacterium additionally has increased activity of CODH/ACS compared to the parental bacterium.
16. The method of claim 15 , wherein the bacterium overexpresses a CODH/ACS gene compared to the parental bacterium.
17. The method of claim 11 , wherein the product comprises one or more of ethanol and 2,3-butanediol.
18. The method of claim 11 , wherein the bacterium produces a higher amount of ethanol, produces a lower amount of acetate, has a shorter lag phase, and/or has a higher growth rate compared to the parental bacterium.
19. The method of claim 11 , wherein the parental bacterium is Clostridium autoethanogenum, Clostridium ljungdahlii , or Clostridium ragsdalei.