IL-15-based molecules and methods of use thereof
View Patent ↗The invention features combination therapies using an IL-15-based superagonist complex and an antibody to effectively treat subjects with cancer and infectious diseases.
1. A method for treating a neoplasia or a human immunodeficiency virus (HIV) infection in a subject, the method comprising:
administering to said subject an effective amount of an antibody and an effective amount of a pharmaceutical composition comprising an IL-15N72D:IL-15RαSu/Fc complex (ALT-803), wherein said antibody is an immune checkpoint inhibitor antibody or a tumor-specific antibody, and wherein said ALT-803 comprises a dimeric IL-15RαSu/Fc and two IL-15N72D molecules,
thereby treating the neoplasia or HIV infection.
2. The method of claim 1 , wherein the IL-15N72D molecule comprises the amino acid sequence of SEQ ID NO: 3.
3. The method of claim 1 , wherein the IL-15RαSu/Fc comprises the amino acid sequence of SEQ ID NO: 6.
4. The method of claim 1 , wherein the subject is suffering from a neoplasia and the neoplasia is selected from the group consisting of a glioblastoma, prostate cancer, hematological cancer, B-cell neoplasms, multiple myeloma, B-cell lymphoma, Hodgkin's lymphoma, chronic lymphocytic leukemia, acute myeloid leukemia, cutaneous T-cell lymphoma, T-cell lymphoma, a solid tumor, urothelial/bladder carcinoma, melanoma, lung cancer, renal cell carcinoma, breast cancer, gastric and esophageal cancer, head and neck cancer, colorectal cancer, ovarian cancer, non-small cell lung carcinoma, B cell non-Hodgkin lymphoma, and squamous cell head and neck carcinoma.
5. The method of claim 1 , wherein the effective amount of said ALT-803 is administered daily.
6. The method of claim 5 , wherein the effective amount of said ALT-803 is between 0.1 μg/kg and 100 mg/kg.
7. The method of claim 1 , wherein the effective amount of said ALT-803 is administered once or twice per week.
8. The method of claim 7 , wherein the effective amount of said ALT-803 is between 0.1 μg/kg and 1 mg/kg.
9. The method of claim 1 , wherein the pharmaceutical composition is administered systemically, intravenously, subcutaneous, intramuscularly, intravesically, or by instillation.
10. The method of claim 1 , wherein said antibody comprises an anti-gp75 antibody, an anti-CD20 antibody, an anti-HER2 antibody, an anti-EGFR antibody, an anti-cytotoxic T-lymphocyte antigen 4 (CTLA-4) antibody, an anti-programmed cell death-1 (PD-1) antibody, an anti-programmed cell death-ligand 1 (PD-L1) antibody, or an anti-programmed cell death-ligand 2(PD-L2) antibody.
11. The method of claim 1 , wherein antibody-dependent cell-mediated cytotoxicity (ADCC) or antibody dependent cellular phagocytosis (ADCP) mediated by said antibody against tumor or infected cells in said subject is augmented by at least 5% following said administration.
12. The method of claim 1 , wherein said antibody and said ALT-803 increases levels of blood NK cell, T cell, neutrophil or monocyte counts or activity.
13. The method of claim 1 , wherein said antibody and said ALT-803 stimulate CD4+ or CD8+ T cells to kill tumor or infected cells.
14. The method of claim 1 , wherein said antibody and said ALT-803 stimulate NK cells to kill tumor or infected cells.
15. The method of claim 1 , wherein said antibody and said ALT-803 stimulate neutrophils or monocytic cells to kill tumor or infected cells.
16. The method of claim 1 , wherein said administration results in a decrease in the number of tumor or infected cells.
17. The method of claim 1 , wherein said administration results in a decrease in disease progression of the neoplasia or infection.
18. The method of claim 1 , wherein said administration results in prolonged survival of said subjects compared to untreated subjects.
19. The method of claim 1 , wherein said subject is a human.
20. The method of claim 1 , wherein said antibody and said ALT-803 are administered simultaneously or sequentially.
21. The method of claim 1 , wherein said administration prevents future recurrence of said neoplasia or infection.
22. The method of claim 1 , wherein said antibody and said ALT-803 increase blood cytokine levels.
23. The method of claim 22 , wherein said cytokine comprises IFN-γ and/or IL-6.
24. The method of claim 1 wherein the subject is suffering from a neoplasia and the neoplasia is treated.
25. The method of claim 1 wherein the subject is suffering from an HIV infection and the HIV infection is treated.