Human serum albumin binding compounds and fusion proteins thereof
The present invention relates to a polypeptide binding to human serum albumin and comprising or consisting of an amino acid sequence selected from the group consisting of: (a) GVTLFVALYDY(X 1 )(X 2 )(X 3 )(X 4 )(X 5 ) (X 6 )D(X 7 )SFHKGEKFQIL(X 8 )(X 9 )(X 10 )(X 11 )(X 12 )G(X 13 )(X 14 )W(X 15 )(X 16 )RSLTTG(X 17 )(X 18 )G(X 19 )IPSNYVAPVDSIQ (SEQ ID NO: 1), wherein (X 1 ) is A, V, I, L, M, G, P, S, T, N, Q, C, R, H, K, D or E; (X 2 ) is R, H, K, A, V, I, L, M, G, P, S, T, N, Q or C; (X 3 ) is R, H, K, S, T, N, Q, C, F, Y, W, A, V, I, L, M, G or P; (X 4 ) is S, T, N, Q, C, A, V, I, L, M, G, P, R, H, K, F, Y, W, D or E; (X 5 ) is S, T, N, Q, C, D, E, F, Y, W, A, V, I, L, M, G, P, R, H or K; (X 6 ) is F, Y, W, A, V, I, L, M, G, P, R, H, K, S, T, N, Q or C; (X 7 ) is A, V, I, L, M, G, P, R, H or K; (X 8 ) is S, T, N, Q, C, D or E; (X 9 ) is S, T, N, Q, C, D, E, A, V, I, L, M, G, P, F, Y or W; (X 10 ) is A, V, I, L, M, G or P; (X 11 ) is F, Y, W, R, H or K; (X 12 ) is S, T, N, Q, C, F, Y or W; (X 13 ) is F, Y, W, R, H, K, S, T, N, Q, C, D, E, A, V, I, L, M, G or P; (X 14 ) is F, Y, W, A, V, I, L, M, G or P; (X 15 ) is D, E, A, V, I, L, M, G or P; (X 16 ) is A, V, I, L, M, G or P; (X 17 ) is D, E, A, V, I, L, M, G, P, R, H or K; (X 18 ) is S, T, N, Q, C, A, V, I, L, M, G or P; (X 19 ) is F, Y, W, S, T, N, Q or C; and (b) an amino acid sequence which is at least 90% identical to the amino acid sequence of (a), wherein the identity determination excludes amino acid positions (X 1 ) to (X 19 ).
1. A polypeptide binding to human serum albumin, wherein the polypeptide comprises an amino acid sequence selected from the group consisting of: SEQ ID Nos: 4 to 40.
2. The polypeptide of claim 1 , wherein the amino acid sequence is selected from the amino acid sequences of SEQ ID NOs: 4, 5 or 6 to 32.
3. A fusion protein comprising the polypeptide of any one of claims 1 or 2 fused to a pharmaceutically and/or diagnostically active protein or peptide.
4. The fusion protein of claim 3 , wherein the polypeptide is directly fused to the pharmaceutically and/or diagnostically active protein or peptide.
5. The fusion protein of claim 3 , wherein the polypeptide is fused to the pharmaceutically and/or diagnostically active protein or peptide via a linker.
6. The fusion protein of claim 3 , wherein the pharmaceutically and/or diagnostically active protein or peptide is selected from the group consisting of a recombinant protein, an antibody, a blood factor, a hormone, an anticoagulans, a thrombolytic, a cytokine, a chemokine, and an interferon.
7. The fusion protein of claim 5 , wherein the antibody is a bispecific T-cell engaging antibody.
8. A pharmaceutical and/or diagnostic composition comprising the fusion protein of claim 3 .