Diagnosis and treatment of viral diseases
Provided are methods of diagnosing a viral disease such as idiopathic pulmonary fibrosis, Castleman's disease, a lymphoma, a thymoma or a sarcoma in a patient by identifying one or more virus-specific elements such as a nucleic acid or a viral protein or a patient antibody to a virus-specific element, as well as to kits for diagnosing the viral disease in a patient. Further provided are methods of monitoring disease progression and/or the efficacy of therapy by measuring the levels of a virus-specific element in a sample from a patient, and methods of identifying therapeutic agents that show efficacy in reducing levels of virus-specific agents in vitro. Still further provided are methods of treating idiopathic pulmonary fibrosis, a lymphoproliferative disease and cancer, as well as methods of preventing viral infection, including Herpesvirus saimiri infection.
1. A method of treating or preventing a disease associated with an infection of Herpesvirus saimiri comprising:
administering to a human patient in need of treatment or prevention of the disease a therapeutically effective amount of an antibody that binds to and neutralizes human IL-17 (SEQ ID NO: 1) or Herpesvirus saimiri IL-17 (SEQ ID NO: 2),
wherein said disease is idiopathic pulmonary fibrosis.
2. The method of claim 1 , before said administering step the steps of:
(a) providing
(i) a human clinical sample from said patient, wherein said patient is suspected of having idiopathic pulmonary fibrosis associated with a Herpesvirus saimiri viral infection,
(ii) a labeled nucleic acid probe comprising one or more sequences derived from Herpesvirus saimiri,
(b) contacting said clinical sample (i) with said labeled nucleic acid probe (ii),
(c) allowing hybridization to take place between said labeled nucleic acid probe (ii) and viral sequences in said clinical sample (i) if present, and
(d) detecting hybridization of said nucleic acid probe (ii) to said viral sequences in said clinical sample (i), thereby providing a diagnosis of said patient as having idiopathic pulmonary fibrosis associated with a Herpesvirus saimiri viral infection.
3. The method of claim 1 , further comprising before said administering step the steps of:
(a) providing
(i) a human clinical sample from said patient wherein said patient is suspected of having idiopathic pulmonary fibrosis associated with a Herpesvirus saimiri viral infection,
(ii) one or more antibodies to viral proteins expressed by Herpesvirus saimiri , said proteins selected from the group consisting of Herpesvirus saimiri DHFR, cyclin D, IL-17 and thymidylate synthase,
(b) contacting said clinical sample (i) with said one or more antibodies (ii),
(c) allowing binding to take place between said one or more antibodies (ii) and said viral proteins in said clinical sample (i), and
(d) detecting the binding of said one or more antibodies (ii) to said viral proteins in the clinical sample (i), thereby providing a diagnosis of said patient as having idiopathic pulmonary fibrosis associated with a Herpesvirus saimiri viral infection.
4. The method of claim 2 , wherein said one or more sequences derived from Herpesvirus saimiri comprise mRNA, DNA, U rich non-coding RNA, or a combination thereof.
5. The method of claim 2 , further comprising a nucleic acid amplification step.
6. The method of claim 3 , wherein the one or more antibodies to viral proteins expressed by Herpesvirus saimiri comprise antibodies to at least two viral proteins selected from the group consisting of DHFR, cyclin D, IL-17 and thymidylate synthase.
7. The method of claim 1 , wherein said antibody binds to and neutralizes Herpesvirus saimiri viral IL-17.