Crystallized oxalate decarboxylase and methods of use
Pharmaceutical compositions comprising spray-dried oxalate decarboxylase crystals are disclosed. Methods to treat a disorder associated with elevated oxalate concentration using compositions comprising spray-dried oxalate decarboxylase crystals are also disclosed.
1. A pharmaceutical composition comprising a capsule containing crystals of spray-dried oxalate decarboxylase comprising the amino acid sequence of SEQ ID NO:1 where the thiol moiety at Cys383 is protected by a reaction with a thiol protecting group, wherein the crystals have a Hausner ratio of about 1.12 to about 1.34, and the capsule comprises at least 3,000 units of oxalate decarboxylase.
2. The pharmaceutical composition of claim 1 , further comprising an excipient.
3. The pharmaceutical composition of claim 2 , wherein the excipient is a sugar.
4. The pharmaceutical composition of claim 3 , wherein the sugar is selected from the group consisting of trehalose, sucrose, and glucose.
5. The pharmaceutical composition of claim 4 , wherein the sugar is trehalose.
6. The pharmaceutical composition of claim 1 , wherein the capsule is a size 1 capsule.
7. A method of reducing oxalate in a mammal, the method comprising administering the pharmaceutical composition of claim 1 to the mammal in an amount sufficient to reduce the level of oxalate in the mammal.
8. The method of claim 7 , wherein the mammal suffers from a disorder selected from the group consisting of primary hyperoxaluria, enteric hyperoxaluria, idiopathic hyperoxaluria, ethylene glycol poisoning, cystic fibrosis, inflammatory bowel disease, urolithiasis, nephrolithiasis, chronic kidney disease, hemodialysis, gastrointestinal bypass, and kidney stones.