Isolation of nucleic acids
Improved compositions for and methods of processing and analyzing samples are described. In particular, the compositions and methods liberate nucleic acids from a biological sample allowing direct downstream processing of the nucleic acids in microfluidic systems. These compositions, methods and kits are useful in diagnosing, staging or otherwise characterizing various biological conditions.
1. A method for releasing nucleic acids contained in a biological sample, the method comprising the step of:
contacting the biological sample with a composition for converting at least part of the sample into a lysate containing said nucleic acids, said lysate being directly transportable through a microfluidic system,
wherein the sample is a wax-embedded sample or a formalin fixation and paraffin embedding (FFPE) sample,
wherein the composition is a liquefaction composition that includes at least a non-ionic surfactant; and
performing a downstream analysis of a nucleic acid potentially present among the nucleic acids contained in the lysate,
wherein the downstream analysis
is performed within the microfluidic system,
comprises an amplification reaction of the nucleic acid, and
is a direct analysis of said nucleic acid, comprising providing at least a portion of the lysate containing the nucleic acids into the amplification reaction without purification of the nucleic acids from the non-ionic surfactant included in the liquefaction composition.
2. The method according to claim 1 wherein the amplification reaction comprises thermocycling.
3. The method according to claim 2 wherein the thermocycling comprises PCR.
4. A method according to claim 1 wherein the nucleic acid is DNA or RNA.
5. The method according to claim 1 wherein the non-ionic surfactant has the formula
R—O—(CH 2 CH 2 O) n H
wherein n>7; n≥8; or n=8;
and/or
R comprises 12≤C≤38.
6. The method according to claim 5 wherein R is CH 3 (CH 2 ) 7 —CH═CH—(CH 2 ) 8 or (CH 2 ) 10 —CH—(CH 3 ) 2 .
7. The method according to claim 5 wherein the non-ionic surfactant is (Z)-3,6,9,12,15,18,21,24-Octaoxadotetracont-33-en-1-ol.
8. A composition for releasing nucleic acid from and for liquefying a wax-embedded or an FFPE biological sample, said composition:
comprising at least a non-ionic surfactant, wherein the non-ionic surfactant is (Z)-3,6,9,12,15,18,21,24-Octaoxadotetracont-33-en-1-ol or polyethylene glycol monoalkyl ether that has the formula R—O—(CH 2 CH 2 O) n H wherein n=8; and
wherein the amount of the non-ionic surfactant is adapted such that when said composition is brought in contact with the biological sample, at least a part of the biological sample is converted into a lysate directly transportable through a microfluidic system, wherein the amount of the non-ionic surfactant in the composition is between 0.10% and 0.40%, said lysate at the same time being suitable for performing a direct analysis of the contained therein released nucleic acid by means of a nucleic acid amplification reaction, wherein said amplification reaction is performed in the presence of said lysate without prior purification of the released nucleic acid from the non-ionic surfactant.
9. A composition for releasing nucleic acid according to claim 8 wherein the non-ionic surfactant is (Z)-3,6,9,12,15,18,21,24-Octaoxadotetracont-33-en-1-ol.
10. A method according to claim 1 wherein the direct analysis comprises providing at least a portion of the lysate into the amplification reaction, wherein the lysate is undiluted or minimally diluted.
11. A method according to claim 3 wherein the PCR comprises real-time detection.
12. A composition for releasing nucleic acid according to claim 8 wherein said lysate is suitable for performing a direct analysis of the released nucleic acid while being undiluted or minimally diluted.
13. A composition for releasing nucleic acid according to claim 8 wherein said lysate is suitable for performing a direct analysis of the released nucleic acid while being diluted 2-fold.
14. The method according to claim 5 wherein the non-ionic surfactant is polyethylene glycol monoalkyl ether.
15. A composition for releasing nucleic acid according to claim 8 wherein the non-ionic surfactant is polyethylene glycol monoalkyl ether.