Cyanobacteria having improved photosynthetic activity
This disclosure describes modified photosynthetic microorganisms, including Cyanobacteria, that have a reduced amount of a light harvesting protein (LHP) and contain one or more introduced or overexpressed polynucleotides encoding one or more enzymes associated with lipid biosynthesis, and which are capable of producing increased amounts of fatty acids and/or synthesizing triglycerides.
1. A cell culture comprising modified Cyanobacteria having a genetic modification including at least one of:
addition of an endogenous NbIA gene, addition of an exogenous NbIA gene, or addition of a promoter that regulates NbIA gene expression, wherein the genetic modification results in changes that, as compared to corresponding wild-type Cyanobacteria, include:
an increased level of NbIA polypeptide or a biologically active fragment of NbIA polypeptide that includes a conserved domain,
an enhanced level of photosynthetic activity, and
a reduced amount of a light harvesting protein (LHP).
2. The cell culture of claim 1 , wherein the modified cyanobacteria grow, divide or both at an increased rate as compared to the corresponding wild-type Cyanobacteria.
3. The cell culture of claim 1 , wherein the modified Cyanobacteria have at least one of:
a reduced amount of phycobilisomes as compared to the corresponding wild-type Cyanobacteria; or
an increased proteolytic degradation of phycobilisomes as compared to the corresponding wild-type Cyanobacteria.
4. The cell culture of claim 1 , wherein the modified Cyanobacteria have at least one of:
an enhanced expression of the endogenous NbIA gene as compared to the corresponding wild-type Cyanobacteria;
a reduced level of a photosystem II-associated light harvesting protein as compared to the corresponding wild-type Cyanobacteria; or
a reduced amount of phycobiliproteins as compared to the corresponding wild-type Cyanobacteria.
5. The cell culture of claim 1 , wherein the genetic modification comprises replacement of a wild-type promoter for the endogenous NbIA gene with a different promoter.
6. The cell culture of claim 1 , wherein the photosynthetic activity is measured based on at least one of a growth rate, a level of oxygen evolution, or a biomass accumulation rate.
7. The cell culture of claim 1 , wherein a growth rate of the modified Cyanobacteria is at least about 120% of a growth rate of the corresponding wild-type Cyanobacteria.
8. A method for generating modified Cyanobacteria, comprising:
modifying a Cyanobacteria by adding an endogenous NbIA gene, adding an exogenous NbIA gene, or adding a promoter that regulates NbIA gene expression thereby:
increasing a level of a NbIA polypeptide or a biologically active fragment of the NbIA polypeptide that includes a conserved domain, as compared to a corresponding wild-type Cyanobacteria,
enhancing a level of photosynthetic activity as compared to the corresponding wild-type Cyanobacteria, and
reducing an amount of a light harvesting protein (LHP) as compared to the corresponding wild-type Cyanobacteria.
9. The method of claim 8 , wherein the modified cyanobacteria grow, divide, or both at an increased rate as compared to the corresponding wild-type Cyanobacteria.
10. The method of claim 8 , wherein the modified Cyanobacteria have at least one of:
a reduced amount of phycobilisomes as compared to the corresponding wild-type Cyanobacteria; or
an increased proteolytic degradation of phycobilisomes as compared to the corresponding wild-type Cyanobacteria.
11. The method of claim 8 , wherein the modified Cyanobacteria have at least one of:
an enhanced expression of the endogenous NbIA gene as compared to the corresponding wild-type Cyanobacteria;
a reduced level of a photosystem II-associated light harvesting protein as compared to the corresponding wild-type Cyanobacteria; or
a reduced amount of phycobiliproteins as compared to the corresponding wild-type Cyanobacteria.
12. The method of claim 8 , wherein adding the promoter comprises replacing a wild-type promoter for the endogenous NbIA gene with a different promoter.
13. The method of claim 8 , wherein the photosynthetic activity is measured based on at least one of a growth rate, a level of oxygen evolution, or a biomass accumulation rate.
14. A modified Cyanobacterium comprising a genetic modification including at least one of:
addition of an endogenous NbIA gene, addition of an exogenous NbIA gene, or addition of a promoter that regulates NbIA gene expression, wherein the genetic modification results in changes that, as compared to a corresponding wild-type Cyanobacterium, include:
increased levels of NbIA polypeptide or a biologically active fragment of NbIA polypeptide that includes a conserved domain domain,
an enhanced level of photosynthetic activity, and
a reduced amount of a light harvesting protein (LHP).
15. The modified Cyanobacterium of claim 14 , wherein the modified Cyanobacterium grow, divide, or both at an increased rate as compared to the corresponding wild-type Cyanobacterium.
16. The modified Cyanobacterium of claim 14 , wherein the modified Cyanobacterium has at least one of:
a reduced amount of phycobilisomes as compared to the corresponding wild-type Cyanobacterium; or
an increased proteolytic degradation of phycobilisomes as compared to the corresponding wild-type Cyanobacterium.
17. The modified Cyanobacterium of claim 14 , wherein the modified Cyanobacterium has at least one of:
an enhanced expression of the endogenous NbIA gene as compared to the corresponding wild-type Cyanobacteria;
a reduced level of a photosystem II-associated light harvesting protein as compared to the corresponding wild-type Cyanobacteria; or
a reduced amount of phycobiliproteins as compared to the corresponding wild-type Cyanobacteria.
18. The modified Cyanobacterium of claim 14 , wherein the addition of the promoter that regulates NbIA gene expression comprises replacement of a wild-type promoter for the endogenous NbIA gene with a different promoter.
19. The modified Cyanobacterium of claim 14 , wherein the photosynthetic activity is measured based on at least one of a growth rate, a level of oxygen evolution, or a biomass accumulation rate.
20. The cell culture of claim 5 , wherein the different promoter comprises an inducible promoter or a constitutive promoter.
21. The method of claim 12 , wherein the different promoter comprises an inducible promoter or a constitutive promoter.
22. The modified Cyanobacterium of claim 14 , wherein a growth rate of the modified Cyanobacterium is at least about 120% of a growth rate of the corresponding wild-type Cyanobacterium.
23. The modified Cyanobacterium of claim 18 , wherein the different promoter comprises an inducible promoter or a constitutive promoter.