Methods and solutions for rapidly killing or deactivating spores
View Patent ↗Exemplary methods and systems for killing or deactivating spores include applying a fluid to a surface containing a spore; and applying direct or indirect plasma to the surface for a period of time. In some embodiments, the fluid includes water. In some embodiments, the spore is Clostridium difficile and in some is Bacillus Anthracis . In some embodiments, the fluid is in the form of a vapor, a fog, a spray, a mist or an aerosol, which may be activated prior to or after applying the fluid to a surface. In some embodiments, peroxynitrite is created in the fluid during the method. Another exemplary embodiment of killing or deactivating a spore includes treating spores with direct plasma or indirect plasma for a period of time and applying an antimicrobial to the spores. In some embodiments, the antimicrobial is an alcohol, a bleach or an alcohol-based sanitizer.
1. A method of killing or deactivating spores comprising:
passing a mixture of one or more gases and droplets of a liquid through a non-thermal plasma to activate the gas/droplet mixture;
applying the activated gas/droplet mixture onto a surface containing one or more spores for a period of time;
wherein the distance between the non-thermal plasma and the surface is such that the gas/droplet mixture passing through the non-thermal plasma reaches the surface in less than about 3 times the half-life of short lived reactive species generated as the gas/droplet mixture passes through the plasma wherein at least some of the short lived reactive species is peroxynitrite.
2. The method of claim 1 wherein the period of time is a function of the time required to kill or deactivate a desired spore.
3. The method of claim 1 wherein the period of time is less than about 1 minute.
4. The method of claim 1 wherein the period of time is less than about 30 seconds.
5. The method of claim 1 wherein the period of time is less than about 10 seconds.
6. The method of claim 1 wherein the distance is such that droplets passing through the non-thermal plasma reach the surface in less than about the half-life of the short lived reactive species.
7. The method of claim 1 wherein the one or more gases comprises air.
8. The method of claim 1 wherein the one or more gases comprises at least one noble gas.
9. The method of claim 1 wherein the liquid comprises water.
10. The method of claim 1 wherein the liquid comprises an additive.
11. The method of claim 1 wherein the additive comprises one of ethanol, acidified ethanol, n-propanol and isopropanol.
12. A method of killing or deactivating spores comprising:
passing a mixture of one or more gases and droplets of a fluid through a non-thermal plasma to activate the gas/droplet mixture and
applying the activated gas/droplet mixture onto a surface containing one or more spores for a period of time that is less than about 1 minute wherein the distance between the non-thermal plasma and the surface is such that the gas/droplet mixture passing through the non-thermal plasma reaches the surface in less than about 3 times the half-life of short lived reactive species generated as the gas/droplet mixture passes through the plasma; wherein at least some of the short lived reactive species is peroxynitrite; wherein the gas/droplet mixture comprises one or more electrostatically charged droplets that cause the droplets to tend to form uniform droplet distribution on the surface to be disinfected.
13. The method of claim 12 wherein the period of time is less than about 30 seconds.
14. The method of claim 12 wherein the period of time is less than about 10 seconds.
15. The method of claim 12 wherein the period of time is less than about 5 seconds.
16. The method of claim 12 wherein after the gas/droplet mixture passes through the non-thermal plasma, the gas/droplet mixture contacts the surface within about 3 seconds.
17. The method of claim 12 wherein after the gas/droplet mixture passes through the non-thermal plasma gas, the gas/droplet mixture contacts the surface within about 2 seconds.
18. The method of claim 12 wherein after the gas/droplet mixture passes through the non-thermal plasma gas, the gas/droplet mixture contacts the surface within about 1 second.