Compositions of and methods for cancellous bone matrix
View Patent ↗A bone repair composition including a cancellous bone matrix with cortical and/or cancellous bone particles loaded therein; and a kit comprising the cancellous bone matrix and the cortical and/or cancellous bone particles.
1. A method of making a monolithic bone implant composition, comprising:
hydrating a cancellous bone matrix to form a swollen cancellous bone matrix;
loading demineralized bone particles into the swollen cancellous bone matrix to form a loaded cancellous bone matrix; and
dehydrating the loaded cancellous bone matrix to form a monolithic bone implant composition comprising a cancellous bone matrix with demineralized bone particles therein.
2. The method of claim 1 , wherein the hydrating comprises a solution selected from the group consisting of water, saline, glycerol, lecithin, sucrose, and combinations thereof.
3. The method of claim 1 , wherein the demineralized bone particles comprise demineralized bone particles in a solution selected from the group consisting of water, saline, alcohol, glycerol, and combinations thereof.
4. The method of claim 1 , wherein the dehydrating comprises heating the loaded cancellous bone matrix at a temperature in a range from about 40° C. to about 75° C.
5. The method of claim 1 , wherein the dehydrating comprises applying a vacuum or reduced pressure and/or heating to the loaded cancellous bone matrix.
6. The method of claim 1 , wherein the demineralized bone particles comprise ethanol.
7. The method of claim 1 , further comprising loading a hypotonic solution into the loaded cancellous bone matrix to swell the demineralized bone particles.
8. The method of claim 1 , further comprising loading an additive into the swollen cancellous bone matrix.
9. The method of claim 1 , wherein the demineralized bone particles comprise an additive.
10. The method of claim 9 , wherein the additive is selected from the group consisting of bone marrow cells, mesenchymal stem cells, oxygenating materials, growth factors, antibiotics, anti-neoplastic agents, and combinations thereof.
11. The method of claim 1 , wherein the hydrating comprises rehydrating a dehydrated cancellous bone matrix.
12. A monolithic bone implant composition made by the method of claim 1 .