Compositions and methods for obtaining enriched mesenchymal stem cell cultures
The disclosure provides a method of culturing cells of the mesenchymal cell lineage, said method comprising contacting the cells with a culture media comprising a CSF1R kinase inhibitor. The disclosure also provides a method of culturing cells from bone marrow and/or compact bone to enrich the cells with cells of the mesenchymal cell lineage comprising contacting the cells with a culture media comprising a CSF1R kinase inhibitor. Cell culture media comprising a CSF1R kinase inhibitor and useful for culturing cells of the mesenchymal cell lineage and/or enriching cells of the mesenchymal cell lineage is also provided.
1. A method of increasing the number of mesenchymal cell in a sample comprising cells of the mesenchymal cell lineage and non-mesenchymal cells, said method comprising contacting the sample with a culture media comprising a Colony Stimulating Factor 1 Receptor (CSF1R) kinase inhibitor to increase the number of mesenchymal cells, wherein the method does not require removal of the non-mesenchymal cells prior to adding the CSF1R kinase inhibitor.
2. The method of claim 1 , wherein the method comprises:
a) harvesting cells from a tissue sample obtained from a subject, wherein the harvested cells comprise cells of the mesenchymal cell lineage and non-mesenchymal cells, and
b) contacting the harvested cells with a culture media comprising a CSF1R kinase inhibitor, and optionally
c) obtaining a population of cells enriched for cells of the mesenchymal cell lineage.
3. The method of claim 2 , wherein the tissue sample comprises bone marrow, compact bone or adipose tissue.
4. The method of claim 1 , wherein the CSF1R kinase inhibitor is GW2580, KI20227, HY-13075, cFMS Receptor Inhibitor II, cFMS Receptor Inhibitor III, cFMS Receptor Inhibitor IV or ARRY-382.
5. The method of claim 1 , wherein the cells of the mesenchymal cell lineage comprise at least one of a mesenchymal stem cell, a mesenchymal cell progenitor and a stromal-derived cell.
6. The method of claim 1 , wherein the cells are contacted with the culture media for at least one hour, at least one day or at least one week.
7. The method of claim 1 , wherein prior to contacting the cells with the culture media, the concentration of the cells of the mesenchymal cell lineage is at least 10 cells/cm 2 .
8. The method of claim 1 , wherein the cells of the mesenchymal cell lineage retain their ability to form adipogenic, chrondrogenic and osteogenic cell lineages.