Bone marrow adipose portion isolation device and methods
The embodiments disclosed herein generally relate to systems, devices and methods for the fractionation, isolation, extraction and processing of the adipose supernatant layer of a bone marrow aspirate. In particular, the various embodiments relate to systems devices and methods of obtaining, utilizing and processing the adipose supernatant layer of a bone marrow aspirate as a source of mesenchymal stem cells.
1. A device for processing bone marrow aspirate comprising:
a first chamber providing for the fractionation of bone marrow aspirate into layers including an adipose layer supernatant;
a second chamber in fluid communication with the first chamber providing for the decanting of the adipose layer supernatant from the first chamber to the second chamber; and
a mechanical emulsification system in fluid communication with the second chamber for emulsifying the adipose layer supernatant.
2. The device for processing bone marrow aspirate of claim 1 wherein the second chamber comprises at least one of a syringe, pipette or tube in fluid communication with the adipose layer supernatant.
3. The device for processing bone marrow aspirate of claim 1 wherein the first chamber comprises one or more of:
a cap comprising a fluid access port providing for the second chamber to be placed into fluid communication with the adipose layer supernatant;
a plunger providing for the expulsion of a selected portion of the fractionated bone marrow aspirate from the first chamber;
a disk shaped volume which provides for the collection of an adipose layer supernatant fraction at a central region of the disk shaped volume upon the rotation of the first chamber around a central axis;
a portion of restricted diameter positioned to correspond with the location of an adipose layer supernatant fraction upon fractionation of bone marrow aspirate placed within the first chamber;
a floating disk having a density selected to cause the disk to float substantially between a serum layer and an adipose layer supernatant fraction upon fractionation of the bone marrow aspirate;
a porous lipophilic membrane providing for the separation of an adipose layer supernatant fraction upon fractionation of the bone marrow aspirate; and
one or more ports in fluid communication with an adipose layer supernatant fraction upon fractionation of the bone marrow aspirate.
4. The device for processing bone marrow aspirate of claim 1 wherein the mechanical emulsification system comprises a first emulsification chamber and a second emulsification chamber in fluid communication with each other through an aperture sized to provide emulsification upon passage of the adipose layer supernatant between the first and second emulsification chambers.
5. The device for processing bone marrow aspirate of claim 1 wherein the mechanical emulsification system comprises a first emulsification chamber and a second emulsification chamber in fluid communication with each other through an emulsification screen providing for the emulsification of the adipose layer supernatant upon passage of adipose layer supernatant between the first and second emulsification chambers.
6. The device for processing bone marrow aspirate of claim 1 wherein the mechanical emulsification system comprises an emulsification screen movable with respect to the adipose layer and providing for the emulsification of the adipose layer supernatant.