Method for producing 5-aminolevulinic acid or salt thereof
View Patent ↗Provided is a method for producing 5-aminolevulinic acid or a salt thereof at a high yield using 5-aminolevulinic acid-producing microorganisms. The method for producing 5-aminolevulinic acid or a salt thereof comprises culturing 5-aminolevulinic acid-producing microorganisms in a medium comprising one or more components selected from the group consisting of L-arginine, glutamic acid, and a salt thereof. The content of glutamic acid or the salt thereof is from 42 to 100 mM in the medium as the glutamic acid.
1. A method for producing 5-aminolevulinic acid or a salt thereof, comprising:
preparing culture medium by adding a yeast extract and separately adding glutamic acid or a salt thereof in an amount of 42 mM to 100 mM as the glutamic acid and L-arginine or a salt thereof in an amount of 0.01 mM to 30 mM as the L-arginine, and
culturing a 5-aminolevulinic acid-producing microorganism in the medium to produce 5-aminolevulinic acid or a salt thereof,
wherein the yeast extract is added in an amount of 5 to 10 g/L.
2. The method according to claim 1 , wherein the L-arginine or the salt thereof is separately added in an amount of 0.5 mM to 15 mM as the L-arginine.
3. The method according to claim 1 , wherein
the 5-aminolevulinic acid-producing microorganism belongs to the genus Rhodobacter.
4. The method according to claim 1 , wherein
the 5-aminolevulinic acid-producing microorganism is Rhodobacter sphaeroides or a variant thereof.
5. The method according to claim 1 , wherein
the 5-aminolevulinic acid-producing microorganism is Rhodobacter sphaeroides CR-0072009 and is deposited under FERM BP-6320.
6. The method according to claim 1 , wherein the glutamic acid or the salt thereof is separately added in an amount of 48 to 80 mM as the glutamic acid.
7. The method according to claim 2 , wherein the glutamic acid or the salt thereof is separately added in an amount of 48 to 80 mM as the glutamic acid.
8. The method according to claim 1 , further comprising adding glycine or levulinic acid to the medium.
9. The method according to claim 1 , further comprising purifying the 5-aminolevulinic acid or the salt thereof.