Hybrid
Provided are transgenic Brassica plants, plant material and seeds, particularly oilseed rape plants, characterized in that these plants harbor a novel combination of two specific transformation events, namely MS-B2 comprising a male-sterility transgene and RF-BN1 comprising a fertility-restoration transgene. Also provided are pairs of Brassica plants comprising each one of the events, and the use thereof in the production of hybrid seed.
1. A method for producing hybrid seed from Brassica oilseed plants, comprising
allowing pollen from a male-fertile male parent Brassica oilseed plant comprising elite event RF-BN1, reference seed comprising said elite event being deposited at the ATCC under deposit number ATCC_PTA-730, to pollinate a male-sterile female parent Brassica oilseed plant comprising elite event MS-B2, reference seed comprising said elite event being deposited at the ATCC under deposit number ATCC_PTA-850; and
harvesting hybrid seed from said female parent plant.
2. The method according to claim 1 , wherein said Brassica oilseed plants belong to the species Brassica napus.
3. A Brassica oilseed plant comprising in its nuclear genome at least one copy of elite event MS-B2, reference seed comprising said elite event being deposited at the ATCC under deposit number ATCC_PTA-850, and at least one copy of elite event RF-BN1, reference seed comprising said elite event being deposited at the ATCC under deposit number ATCC_PTA-730.
4. The Brassica oilseed plant of claim 3 , wherein said Brassica oilseed plant belongs to the species Brassica napus.
5. A cell or tissue or seed of the Brassica oilseed plant of claim 3 , wherein said cell or tissue or seed comprises in its nuclear genome at least one copy of elite event MS-B2, reference seed comprising said elite event being deposited at the ATCC under deposit number or ATCC_PTA-850, and at least one copy of elite event RF-BN1, reference seed comprising said elite event being deposited at the ATCC under deposit number ATCC_PTA-730.
6. A pair of Brassica oilseed plants for use in production of hybrid seed, one of said Brassica oilseed plants comprising elite event MS-B2, reference seed comprising said elite event being deposited at the ATCC under deposit number ATCC_PTA-850, and the other of said Brassica oilseed plants comprising elite event RF-BN1, reference seed comprising said elite event being deposited at the ATCC under deposit number ATCC_PTA-730.
7. Genomic DNA of a Brassica oilseed plant according to claim 3 , comprising at least one copy of elite event MS-B2, reference seed comprising said elite event being deposited at the ATCC under deposit number ATCC_PTA-850, and at least one copy of elite event RF-BN1, reference seed comprising said elite event being deposited at the ATCC under deposit number ATCC_PTA-730.
8. A Brassica juncea plant or plant cell comprising elite event RF-BN1, reference seed comprising said elite event being deposited at the ATCC under deposit number ATCC_PTA-730.
9. Seed from the Brassica juncea plant according to claim 8 , comprising elite event RF-BN1, reference seed comprising said elite event being deposited at the ATCC under deposit number ATCC_PTA-730.
10. The plant or cell according to claim 8 , further comprising elite event MS-B2, reference seed comprising said elite event being deposited at the ATCC under deposit number ATCC_PTA-850.
11. The method of claim 1 , wherein said elite event RF-BN1 is in homozygous form.
12. The method according to claim 1 , wherein said Brassica oilseed plants belong to the species Brassica juncea.
13. The Brassica oilseed plant of claim 3 , wherein said Brassica oilseed plant belongs to the species Brassica juncea.