Probiotic recolonisation therapy
The present invention relates to pharmaceutical compositions suitable for the treatment of chronic diseases associated with the presence of abnormal or an abnormal distribution of microflora in the gastrointestinal tract of a mammalian host, which compositions comprise viable non-pathogenic or attenuated pathogenic Clostridia . The compositions further comprise one or more additional viable non-pathogenic or attenuated pathogenic microorganisms selected from the group consisting of Bacteroides, Eubacteria, Fusobacteria, Propionibacteria, Lactobacilli, anaerobic cocci, Ruminococcus, E. coli, Gemmiger, Desulfomonas, Peptostreptococcus , and fungi. The present invention also provides pharmaceutical compositions suitable for the treatment of the same chronic diseases comprising viable non-pathogenic or attenuated pathogenic Escherichia coli , at least one strain of viable non-pathogenic or attenuated pathogenic Bacteroides and at least one strain of viable non-pathogenic or attenuated pathogenic microorganism.
1. A pharmaceutical composition in an enteric capsule or enteric microcapsule comprising a plurality of viable non-pathogenic Clostridium spores and a plurality of viable non-pathogenic Collinsella , wherein said pharmaceutical composition does not comprise viable Bacteroides , and wherein said plurality of viable non-pathogenic Clostridium spores and said plurality of viable non-pathogenic Collinsella are protected by an enteric coating.
2. The pharmaceutical composition of claim 1 , wherein said pharmaceutical composition does not comprise viable Escherichia coli.
3. The pharmaceutical composition of claim 1 , wherein said pharmaceutical composition does not comprise viable Peptostreptococcus.
4. The pharmaceutical composition of claim 1 , wherein said pharmaceutical composition does not comprise viable Lactobacillus.
5. The pharmaceutical composition of claim 1 , wherein said pharmaceutical composition does not comprise viable Bifidobacterium.
6. The pharmaceutical composition of claim 1 , wherein said viable non-pathogenic Clostridium spores are from a species selected from the group consisting of Clostridium bifermentans, Clostridium innocuum, Clostridium ramosum , and Clostridium butyricum.
7. The pharmaceutical composition of claim 1 , wherein said viable non-pathogenic Collinsella are Collinsella aerofaciens.
8. A pharmaceutical composition in an enteric capsule or enteric microcapsule comprising a plurality of viable non-pathogenic Clostridium spores and a plurality of viable non-pathogenic Collinsella , wherein said pharmaceutical composition does not comprise viable organisms from at least one of the groups consisting of Bacteroides, Fusobacteria, Propionibacteria, Lactobacillus, Ruminococcus, E. coli, Gemmiger, Desulfomonas, Peptostreptococcus , and Bifidobacterium , wherein said enteric capsule or enteric microcapsule protects said pharmaceutical composition from gastric inactivation.
9. The pharmaceutical composition of claim 1 , wherein said enteric capsule or enteric microcapsule comprises a gastric acid suppression agent.
10. The pharmaceutical composition of claim 1 , wherein said pharmaceutical composition recolonises a dysbiotic flora of said gastrointestinal tract.
11. The pharmaceutical composition of claim 8 , wherein said enteric capsule or enteric microcapsule comprises a gastric acid suppression agent.
12. The pharmaceutical composition of claim 8 , wherein said pharmaceutical composition recolonises a dysbiotic flora.
13. A pharmaceutical composition in a capsule or microcapsule adapted for enteric delivery comprising a plurality of viable non-pathogenic Clostridium spores and a plurality of viable non-pathogenic Collinsella , wherein said pharmaceutical composition does not comprise viable Bacteroides.
14. The pharmaceutical composition of claim 13 , wherein said pharmaceutical composition does not comprise viable Escherichia coli.
15. The pharmaceutical composition of claim 13 , wherein said pharmaceutical composition does not comprise viable Peptostreptococcus.
16. The pharmaceutical composition of claim 13 , wherein said pharmaceutical composition does not comprise viable Lactobacillus.
17. The pharmaceutical composition of claim 13 , wherein said pharmaceutical composition does not comprise viable Bifidobacterium.
18. The pharmaceutical composition of claim 13 , wherein said viable non-pathogenic Clostridium spores are from a species selected from the group consisting of Clostridium bifermentans, Clostridium innocuum , and Clostridium butyricum.
19. The pharmaceutical composition of claim 13 , wherein said viable non-pathogenic Collinsella are Collinsella aerofaciens.
20. The pharmaceutical composition of claim 13 , wherein said capsule or microcapsule comprises a gastric acid suppression agent.
21. The pharmaceutical composition of claim 13 , wherein said pharmaceutical composition recolonises a dysbiotic flora.
22. The pharmaceutical composition of claim 1 , wherein said enteric coating is a coating of said capsule or microcapsule.