Probiotic recolonisation therapy
The present invention relates to pharmaceutical compositions suitable for the treatment of chronic diseases associated with the presence of abnormal or an abnormal distribution of microflora in the gastrointestinal tract of a mammalian host, which compositions comprise viable non-pathogenic or attenuated pathogenic Clostridia. The compositions further comprise one or more additional viable non-pathogenic or attenuated pathogenic microorganisms selected from the group consisting of Bacteroides , Eubacteria, Fusobacteria, Propionibacteria, Lactobacilli , anaerobic cocci, Ruminococcus, E. coli , Gemmiger, Desulfomonas, Peptostreptococcus , and fungi. The present invention also provides pharmaceutical compositions suitable for the treatment of the same chronic diseases comprising viable non-pathogenic or attenuated pathogenic Escherichia coli , at least one strain of viable non-pathogenic or attenuated pathogenic Bacteroides and at least one strain of viable non-pathogenic or attenuated pathogenic microorganism.
1. An oral pharmaceutical composition in a capsule or microcapsule adapted for enteric delivery comprising a plurality of viable non-pathogenic or attenuated pathogenic spores of at least two Clostridium species, wherein said oral pharmaceutical composition does not comprise one or more viable microorganisms selected from the group consisting of Bacteroides, Eubacierium Fusobacierium Propionibacterium, Lactobacillus, Ruminococcus, Escherichia coli, Gemmiger, Desulfomonas, Peptostreptococcus, Bifidobacterium , and Monilia.
2. The oral pharmaceutical composition of claim 1 , wherein said capsule or microcapsule further comprises viable Collinsella.
3. The oral pharmaceutical composition of claim 1 , wherein said capsule or microcapsule further comprises viable Bacteroides.
4. The oral pharmaceutical composition of claim 1 , wherein said capsule or microcapsule further comprises viable Escherichia coli.
5. The oral pharmaceutical composition of claim 1 , wherein said capsule or microcapsule further comprises viable Peptostreptococcus.
6. The oral pharmaceutical composition of claim 1 , wherein said capsule or microcapsule further comprises viable Lactobacillus.
7. The oral pharmaceutical composition of claim 1 , wherein said capsule or microcapsule further comprises viable Bifidobacterium.
8. The oral pharmaceutical composition of claim 1 , wherein said capsule or microcapsule further comprises viable Monilia.
9. The oral pharmaceutical composition of claim 8 , wherein said at least two Clostridium species are selected from the group consisting of Clostridium absonum, Clostridium argentinense, Clostridium baratii, Clostridium botulinum, Clostridium butyricum, Clostridium cadaveris, Clostridium carnis, Clostridium celatum, Clostridium chauvoei, Clostridium clostridioforme, Clostridium cochlearium, Clostridium fallax, Clostridium felsineum, Clostridium ghonii, Clostridium glycolicum, Clostridium haemolyticum, Clostridium hastiforme, Clostridium histiolyticum, Clostridium indolis, Clostridium irregulare, Clostridium limosum, Clostridium malenominatum, Clostridium novyi, Clostridium oroticum, Clostridium paraputrificum, Clostridium perferingens, Clostridium piliforme, Clostridium putrefaciens, Clostridium Putrificum, Clostriduim sardiniense, Clostridium sartagoforme, Clostridium scindens, Clostridium septicum, Clostridium sordellii, Clostridium sphenoides, Clostridium spiroforme, Clostridium sporogenes, Clostridium subterminale, Clostridium symbiosum, Clostridium tertium, Clostridium tetani, Clostridium welchii, and Clostridium villosum.
10. The oral pharmaceutical composition of claim 1 , wherein said at least two Clostridium species are selected from the group consisting of Clostridium bifermentans, Clostridium innocuum, Clostridium ramosum , and Clostridium butyricum.
11. The oral pharmaceutical composition of claim 1 , wherein said oral pharmaceutical composition comprises between about 10 3 and about 10 13 viable non-pathogenic or attenuated pathogenic spores or cells.
12. The oral pharmaceutical composition of claim 1 , wherein said oral pharmaceutical composition comprises between about 10 5 and about 10 11 viable non-pathogenic or attenuated pathogenic spores or cells.
13. The oral pharmaceutical composition of claim 1 , wherein said oral pharmaceutical composition comprises between about 10 9 and about 10 11 viable non-pathogenic or attenuated pathogenic spores or cells.
14. The oral pharmaceutical composition of claim 1 , wherein said oral pharmaceutical composition comprises about 10 10 viable non-pathogenic or attenuated pathogenic spores or cells.
15. An oral pharmaceutical composition in a capsule or microcapsule adapted for enteric delivery comprising a plurality of viable non-pathogenic or attenuated pathogenic spores of at least two Clostridium species, wherein said oral pharmaceutical composition does not comprise one or more viable microorganisms selected from the group consisting of Bacteroides fraggilis ssp. vulgatus, Collinsella aerofaciens, Bacteroides fragilis ssp. thetaiotaomicron, Peptostreptococcus productus II, Parabacteroides distasonis, Faecalibacterium prausnitzii, Coprococcus eutactus, Peptostreptococcus procluctus , I, Ruminococcus bromii, Byfidobacterium adolescentis, Gemmiger formicilis, Bifidobacterium longum, Eubacterium siraeum, Ruminococcus torques, Eubacterium rectale, Eubacterium leligens, Bacteroides eggerthii, Clostridium leptum, Bacteroides fragilis ssp, A, Eubacterium biforme, Bifidobacterium infantis, Eubacterium rectale Coprococcus comes, Pseudoflavonifractor capillosus, Ruminococcus albus, Dorea formicigenerans, Eubacterium hallii, Eubacterium ventriosum I, Fusobacterium russi, Ruminococcus obeum, Eubacterium rectale, Clostridium ramosum, Lactobacillus leichmannii, Ruminococcus callidus, Butyrivibrio crossotus, Acidaminococcus fermentans, Eubacterium ventriosum, Bacteroides fragilis ssp. fragilis, Bacteroides AR, Coprococcus catus, Aerostipes hadrus, Eubacterium cylindroides, Eubacterium ruminantium, Staphylococcus epidermidis, Eubacterium limosum, Tissirella praeacuta, Fusobacterium mortiferum I, Fusobacterium naviforme, Clostridium innocuum, Clostridium ramosum, Propionibacterium acnes, Ruminococcus flavefaciens, Bacteroides fragilis ssp. ovatus, Fusobacterium nucleatum, Fusobacterium mortiferum, Escherichia coli, Gemella morbillorum, Finegoldia magnus, Streptococcus inteimedius, Ruminococcus lactaris, Eubacterium tenue, Eubacterium ramulus, Bacteroides clostridiiformis ssp. clostridliformis, Bacteroides coagulans, Prevotella oralis, Prevotella ruminicola, Odoribacter splanchnicus, Desuifomanas ,pigra , and any combination thereof.
16. The oral pharmaceutical composition of claim 3 , wherein said capsule or microcapsule further comprises viable Escherichia coli.
17. The oral pharmaceutical composition of claim 1 , wherein said oral pharmaceutical composition does not comprise one or more viable microorganisms selected from the group consisting of Eubacterium , Lactobacillus , and Bifidobacterium.
18. The oral pharmaceutical composition of claim 1 , wherein said oral pharmaceutical composition is for treating a Clostridium difficile infection or for treating autism in a subject further comprising a gastrointestinal symptom selected from the group consisting of irritable bowel syndrome, chronic persistent diarrhoea, diarrhoea, flatulence, constipation, and alternating constipation/diarrhoea.
19. The oral pharmaceutical composition of claim 18 , wherein said Clostridium difficile infection is a recurrent Clostridium difficile infection.
20. The oral pharmaceutical composition of claim 18 , wherein no existing enteric microflora is removed from a subject prior to administration of said oral pharmaceutical composition to said subject.
21. The oral pharmaceutical composition of claim 18 , wherein said oral pharmaceutical composition is administered to a subject pretreated with an antibiotic, wherein said antibiotic is selected from the group consisting of vancomycin, rifampicin, nitroimidazole, and chloramphenicol.
22. The oral pharmaceutical composition of claim 1 , wherein said oral pharmaceutical composition is in an enteric-coated capsule or an enteric-coated microcapsule.
23. The oral pharmaceutical composition of claim 22 , wherein said enteric-coated capsule or said enteric-coated microcapsule comprises a gastric acid suppression agent.
24. The oral pharmaceutical composition of claim 1 , wherein said oral pharmaceutical composition recolonises a dysbiotic intestinal flora.
25. The oral pharmaceutical composition of claim 1 , wherein said oral pharmaceutical composition comprises at least three Clostridium species.
26. A pharmaceutical composition in a capsule or microcapsule, adapted for enter delivery comprising a plurality of viable non-pathogenic or attenuated pathogenic Clostridium spores, a plurality of viable non-pathogenic or attenuated pathogenic Bacteroides , and a plurality of viable non-pathogenic or attenuated pathogenic E. coli , wherein said pharmaceutical composition does not comprise one or more viable microorganism selected from the group consisting of Eubacterium, Fusobacterium, Propionibacterium, Lactobacillus, Ruminococcus, Gemmiger, Desulfomonas, Peptostreptococcus, Bifidobacterium and Monilia.
27. The pharmaceutical composition of claim 26 , wherein said pharmaceutical composition does not comprise one or more viable microorganisms selected from the group consisting of Eubacterium, Lactobacillus , and Bifidobaciterium.
28. The pharmaceutical composition of claim 26 , wherein said pharmaceutical composition does not comprise one or more viable microorganisms selected from the group consisting of Bacteroides fragilis ssp. vulgatus, Collinsella aerofaciens, Bacteroides fragilis ssp. thetaiotaomicron. Peptostreptococcus productus II, Parabacteroides distasonis, Faecalibacterium prausnitzii, Coprococcus eutactus, Peptostreptococcus productus I, Ruminococcus bromii, Bifidobacterium adolescentis, Gemmiger formicilis, Bifidobacterium longum, Eubacterium siraeum, Ruminococcus torques, Eubacterium rectale, Eubacterium eligens, Bacteroides eggerthii, Clostridium leptum, Bacteroides fragilis ssp. A, Eubacterium biforme, Bifidobacterium infantis, Eubacterium rectale , Coprococcus comes , Pseudoflavonifractor capillosus, Ruminococcus albus, Dorea formicigenerans, Eubacterium hallii, Eubacterium ventriosum I, Fusobacterium russi, Ruminococcus obeum, Eubacterium rectale, Clostridium ramosum, Lactobacillus leichmannii, Ruminococcus callidus, Butyrivibrio crossotus, Acidaminococcus fermentans, Eubacterium ventriosum, Bacteroides fragilis ssp. fragilis, Bacteroides AR, Coprococcus catus, Aerostipes hadrus, Eubacterium cylindroides, Eubacterium ruminantium, Staphylococcus epidermidis, Eubacterium limosum. Tissirella praeacuta, Fusobacterium mortiferum I, Fusobacterium naviforme, Clostridium innocuum, Clostridium ramosum, Propionibacterium acnes, Ruminococcus flavefaciens, Bacteroides fragilis ssp. ovatus, Fusobacterium nucleattum, Fusobacterium mortiferum, Escherichia coli, Gemella morbillorum, Finegoldia magnus, Streptococcus intermedius, Ruminococcus lacktaris, Eubacterium tenue, Eubacterium ramulus, Bacteroides clostridiiformis ssp. clostridiiformis, Bacteroides coagulans, Prevotella oralis, Prevotella ruminicola, Odoribacter splanchnicus, Desuifomonas pigra , and any combination thereof.
29. The pharmaceutical composition of claim 26 , wherein said pharmaceutical composition is for treating a Clostridium difficile infection or for treating autism in a subject further comprising a gastrointestinal symptom selected from the group consisting of irritable bowel syndrome, chronic persistent diarrhoea, diarrhoea, flatulence, constipation, and alternating constipation/diarrhoea.
30. The pharmaceutical composition of claim 26 , wherein said pharmaceutical composition is in an enteric-coated capsule or an enteric-coated microcapsule.