Biomass conversion to fuels and chemicals
View Patent ↗This disclosure relates to compositions and methods for converting biomass to various chemical intermediates and final products including fuels. Aspects include the depolymerization of lignin, cellulose, and hemicellulose to a wide slate of depolymerization compounds that can be subsequently metabolized by genetically modified bacterium, and converted to cis,cis-muconic acid. Other aspects include the use of monometallic catalysts for converting the cis,cis-muconic acid to commodity chemicals and fuels, for example adipic acid and/or nylon.
1. A genetically modified prokaryotic microorganism comprising:
an exogenous genetic addition encoding at least a 3-dehydroshikimate dehydratase and a phenol monoxygenase, wherein said genetically modified prokaryotic microorganism is capable of producing cis, cis-muconic acid.
2. The genetically modified prokaryotic microorganism of claim 1 , further comprising:
an endogenous gene deletion that ablates expression of at least one of a protocatechuic acid dioxygenase, a muconate lactonizing enzyme, or a muconolactone isomerase.
3. The genetically modified prokaryotic microorganism of claim 1 , further comprising:
an exogenous genetic addition encoding a vanillic acid demethylase.
4. The genetically modified prokaryotic microorganism of claim 1 , further comprising a deletion of an endogenous catabolite repression control gene.
5. The genetically modified prokaryotic microorganism of claim 1 , further comprising an exogenous genetic addition encoding at least a protocatechuic acid decarboxylase.
6. The genetically modified prokaryotic microorganism of claim 5 , wherein said protocatechuic acid decarboxylase comprises at least one of EcdB or EcdD.
7. The genetically modified prokaryotic microorganism of claim 1 , wherein said 3-dehydroshikimate dehydratase is from Bacillus cereus.
8. The genetically modified prokaryotic microorganism of claim 7 , wherein said 3-dehydroshikimate dehydratase comprises at least one of AroZ or AsbF.
9. The genetically modified prokaryotic microorganism of claim 1 , wherein said phenol monooxygenase is from Pseudomonas sp. CF600.
10. The genetically modified prokaryotic microorganism of claim 9 , wherein said phenol monooxygenase comprises at least one DmpKLMNOP or PheA.
11. The genetically modified prokaryotic microorganism of claim 2 , wherein said protocatechuic acid dioxygenase comprises at least one of PcaH or PcaG.
12. The genetically modified prokaryotic microorganism of claim 2 , wherein at least one of said muconate lactonizing enzyme or said muconolactone isomerase comprises at least one of CatB or CatC.
13. The genetically modified prokaryotic microorganism of claim 3 , wherein said vanillic acid demethylase comprises at least one of VanA, VanB, or LigM.
14. The genetically modified prokaryotic microorganism of claim 1 , wherein the genetically modified prokaryotic microorganism is from the genus Pseudomonas.
15. The genetically modified prokaryotic microorganism of claim 14 , wherein the genetically modified prokaryotic microorganism is P. putida, P. fluorescens , or P. stutzeri.
16. The genetically modified prokaryotic microorganism of claim 15 , wherein said genetically modified prokaryotic microorganism is P. putida KT2440.
17. The genetically modified prokaryotic microorganism of claim 10 , wherein said DmpKLMNOP has an amino acid sequence that is at least 90% identical to the amino acid sequence set forth in SEQ ID Nos: 24, 26, 28, 30, 32, and 34.
18. The genetically modified prokaryotic microorganism of claim 10 , wherein said PheA has an amino acid sequence that is at least 90% identical to the amino acid sequence set forth in SEQ ID NO: 36.
19. The genetically modified prokaryotic microorganism of claim 13 , wherein said LigM has an amino acid sequence that is at least 90% identical to the amino acid sequence set forth in SEQ ID NO: 38.
20. The genetically modified prokaryotic microorganism of claim 11 , wherein said PcaH has an amino acid sequence that is at least 90% identical to the amino acid sequence set forth in SEQ ID NO: 10.
21. The genetically modified prokaryotic microorganism of claim 11 , wherein said PcaG has an amino acid sequence that is at least 90% identical to the amino acid sequence set forth in SEQ ID NO: 12.
22. The genetically modified prokaryotic microorganism of claim 12 , wherein said CatB has an amino acid sequence that is at least 90% identical to the amino acid sequence set forth in SEQ ID NO: 2.
23. The genetically modified prokaryotic microorganism of claim 12 , wherein said CatC has an amino acid sequence that is at least 90% identical to the amino acid sequence set forth in SEQ ID NO: 4.
24. A process comprising:
contacting a culture broth comprising a lignin depolymerization compound with a genetically modified prokaryotic microorganism comprising a first exogenous genetic addition encoding at least a 3-dehydroshikimate dehydratase and a phenol monooxygenase, wherein:
said genetically modified prokaryotic microorganism is capable of converting at least a portion of said lignin depolymerization compound to cis, cis-muconic acid.
25. The process of claim 24 , wherein said lignin depolymerization compound comprises at least one of p-coumaric acid, ferulic acid, benzoic acid, phenol, coniferyl alcohol, caffeic acid, vanillin, or 4-hydroxybenzoic acid.
26. The process of claim 24 , wherein:
said culture broth further comprises a polysaccharide depolymerization compound, and
said genetically modified prokaryotic microorganism is capable of converting at least a portion of said polysaccharide depolymerization compound to cis, cis-muconic acid.
27. The process of claim 24 , wherein said polysaccharide depolymerization compound comprises at least one of glucose, xylose, arabinose, mannose, galactose, or rhamnose.