Evaluating genetic disorders
The present invention relates to genetic analysis and evaluation utilizing copy-number variants or polymorphisms. The methods utilize array comparative genomic hybridization and PCR assays to identify the significance of copy number variations in a human, non-human animal, and plant subject or subject group.
1. A method of hybridizing a nucleic acid probe or synthesizing a nucleic acid product comprising
(a) hybridizing a nucleic acid probe to a polynucleic acid from at least one plant subject with a trait and at least 100 plant subjects without the trait by nucleic acid hybridization or microarray analysis, or
synthesizing a nucleic acid product from a polynucleic acid from at least one plant subject with a trait and each of at least 100 plant subjects without the trait by PCR or sequencing;
wherein the at least one plant subject with the trait and the at least 100 plant subjects without the trait are the same plant species belonging to a family selected from the group consisting of angiosperms, gymnosperms, pteridophytes, and bryophytes; and
(b) detecting at least one copy number variation from the at least one plant subject with the trait that occurs at least once per plant subject with the trait, wherein detecting is detecting by the nucleic acid hybridization, microarray analysis, PCR or sequencing, and wherein a frequency of the copy number variation in the 100 plant subjects without the trait was determined.
2. The method of claim 1 , wherein the at least 100 plant subjects without the trait comprise at least 1,000 plant subjects without the trait.
3. The method of claim 1 , wherein the at least 100 plant subjects without the trait comprise at least 5,000 plant subjects without the trait.
4. The method of claim 1 , wherein the at least 100 plant subjects without the trait comprise at least 10,000 plant subjects without the trait.
5. The method of claim 1 , wherein the whole genome or exome of the at least one plant subject with the trait is analyzed.
6. The method of claim 1 , wherein the whole genome or exome of the at least 100 plant subjects without the trait is analyzed.
7. The method of claim 1 , wherein the trait is a phenotype.
8. The method of claim 1 , wherein the plant species is a plant crop.
9. The method of claim 1 , wherein the plant species is a vascular plant.
10. The method of claim 1 , wherein the plant species is a non-vascular plant.
11. The method of claim 1 , wherein the plant species is selected from the group consisting of corn, wheat, rice, sugar cane, barley, cotton, algae, and seaweed.
12. The method of claim 1 , wherein the plant species is Arabidopsis thaliana.
13. The method of claim 1 , wherein the trait is a growth characteristic.
14. The method of claim 13 , wherein the growth characteristic is associated with resistance to biotic stress, resistance to abiotic stress, adaptive capacity, or any combination thereof.
15. The method of claim 13 , wherein the growth characteristic is associated with an increased or decreased flowering time, plant height, growth rate, or any combination thereof.
16. The method of claim 1 , wherein the number of the at least one copy number variation of each of the at least 100 plant subjects without the trait is none.
17. The method of claim 1 , wherein the number of the at least one copy number variation of each of the at least 100 plant subjects without the trait is a number that is a statistically significant amount less than the number of the at least one copy number variation detected in the at least one plant subject with the trait.
18. The method of claim 1 , wherein the method comprises generating a copy number breakpoint map.
19. The method of claim 1 , wherein the detecting comprises a microarray analysis.
20. The method of claim 19 , wherein the microarray analysis is an array Comparative Genomic Hybridization analysis.
21. The method of claim 1 , wherein the detecting comprises sequencing.
22. The method of claim 1 , wherein the sequencing is a high-throughput sequencing method.
23. The method of claim 1 , wherein the detecting comprises FISH.
24. The method of claim 1 , wherein the detecting comprises PCR.
25. The method of claim 19 , wherein the microarray analysis is an SNP array analysis.
26. The method of claim 1 , wherein the method comprises determining the breakpoints of the at least one copy number variation.
27. The method of claim 26 , wherein the breakpoints are determined by PCR, sequencing, or microarray.